Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
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Questions related to Plasmids
You changed the primers and the water what this could be!!
13 February 2023 6,028 2 View
Hi everyone. I am trying to make point mutations in iPSCs using CRISPR/Cas9. I'm using lipofectamine stem transfection, and then waiting 48 hours before FACS sorting iPSCs (our Cas9 has a GFP) to...
13 February 2023 1,002 5 View
Hi all, once again electroporation of Pichia proves troublesome. I am electroporating GS115 strain with a pPIC9K plasmid. Following three days of growth, both the MD and YPD plates contained pink...
13 February 2023 3,115 3 View
please i need a simple explanation
13 February 2023 2,991 3 View
I'm trying to design a primer for Pestivirus C. I confirmed the potential accessions of this virus in my sample by mNGS and designed 6 universal primer pairs for these acc. These primer pairs...
10 February 2023 4,415 7 View
We are working on vector isolation in the lab, the second time we did the isolation we got a concentration of 50ng/ul which is to lower then desired. The protocol followed siad to use a 1.5 ml...
10 February 2023 4,101 2 View
Hello to dear students, professors and scientists all around the world. I'm Mohammad Reza Eslami. Master of Medical Science student in Medical Biotechnology in Iran University of Medical Science...
10 February 2023 7,080 6 View
Hello. I am to conduct method validation using ELISA. But, I have run into difficulties. How can I conduct specificity and selectivity? Please tell me about mapping.
10 February 2023 6,529 1 View
I have an AAV plasmid and I need to insert a fragment that is 48 bp long. What's the most efficient way to generate the fragment? I have another plasmid that contains the fragment sequence. Can I...
10 February 2023 5,380 3 View
I am conducting a multiplex flow cytometry assay to count the CD3, CD4, CD8 and CD21 markers in bovine PBMCs. However, the manufacturer of CD3 antibody recommends fixing the cells and...
10 February 2023 8,630 2 View
Hi, i was planing to use transfer plasmid and a HIV backbone plasmid containing gag to assessed integrase activity. To do that im missing an information. When i transfect a cell with both plasmid...
09 February 2023 5,957 1 View
I am working with RNA switches for tuning few genes for production of metabolites in Lactococcus. I have observed recombination events in my plasmids when I culture the respective strains for...
09 February 2023 5,583 2 View
For my experiment, I am trying to observe the growth of two gut microbes against in vitro agar substances which represent dietary fibre (turmeric, golden seal, and citric acid) and fats (palmitic...
08 February 2023 5,579 6 View
I am carrying out polymorphism studies using ssr markers and running them on 6% polyacrylamide gel using 1x tbe buffer. But I'm facing smearing type of bands and multiple bands in certain samples...
08 February 2023 5,760 5 View
I used muscle cells as sample, and the western blot band appear grey and blurry. I used non-fat milk for blocking, does this has any effect? This is my 10th time trying and the band still appear...
07 February 2023 4,511 1 View
I am detecting an additional band aside from the expected VP1, VP2, and VP3 bands. This is AAV9, and the extra band is appearing at 100-110 Kda. Ideas?
07 February 2023 8,642 3 View
Hello everyone, I am once again transforming yeast Pichia pastoris after many years and have troubles again. I tried protocol by Kumar (2019) Simplified protocol for faster transformation of (a...
06 February 2023 7,840 2 View
I am curently trying to extract DNA from culture media, i am using the zymoresearch kit for DNA extraction, however i need a blank for the spectophotometric determination. however, I don't...
06 February 2023 9,994 1 View
in transcription process why doesnt need primer for bulid mRNA
04 February 2023 8,534 2 View
transformation after ligation is nessesary step to get desired product in large amount.
04 February 2023 2,873 4 View
I have to use Miniprep and Midiprep for plasmid extraction. But, I need to know what are exact differences and purposes of Mini and Midipreps other than resulting plasmid yield. And, What out of...
01 February 2023 1,494 4 View
I am running HPV PCR with the product size of 450bp. the products are coming at 500 and 464 respectively. also when I run the PCR again with the same conditions, there are no bands visible. I have...
01 February 2023 7,453 3 View
My EMSA results show smears of DNA protein complex instead of band, maybe because of non stable protein DNA complex. I tried reducing salt concentrations, but it didnt work. I have also tried...
31 January 2023 9,135 6 View
I need to insert a 9kb fragment into a 11 kb vector. I planed to use gibson assembly and the enzyme I used was from clonesmarter(Seamless Assembly cloning kit) and Yeasen(Universal One Step...
29 January 2023 7,000 3 View