Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
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Questions related to Plasmids
Hi, I predicted pharmacophore targets with PharmMapper. Can anyone help me to analyze the data? I want to know if the predicted targets are related to my desired gene or not. Is there a way? Thank...
25 October 2023 8,856 2 View
I am would like to know if there is any optimized expression cassette for penecillin or beta lactam antibiotic genes or vancomycin. i have gone through many research paper but most of the...
24 October 2023 7,844 1 View
Hi! I have a bacterial strain that changes the pH of the broth making it acidic. I want to get a knock-out mutant that is unable to lower the pH. Since I'm using transposon mutagenesis without...
24 October 2023 4,652 0 View
Agrobacterium-mediated transformation Particle bombardment
24 October 2023 6,103 0 View
When there are a large number of samples to read OD600 and we want to use a plate reader, how much volume should we add to each well, and does it require dilution? Do you have the complete...
23 October 2023 5,366 4 View
this cell line will serve as the control group. Thanks
21 October 2023 3,619 4 View
Sincere greetings My academic English writing is excellent. I desired to join several active groups within my field. My concentration is in molecular genetics. But I was unable to locate any. I...
21 October 2023 7,598 2 View
How could microplastics contaminate potable water?
20 October 2023 3,424 1 View
I wish to mix CBD into rodent chow as well as WIN 55, 212-2. Because WIN is a schedule 1 drug, I'm not able to have this premixed by a reputable vendor. I was thinking of using either glycerol or...
20 October 2023 7,884 0 View
Hi I used the MTT assay for drug-inhibiting cancer cells Then, I used GraphPad prism to calculate the IC50 of this drug following these equations - log (inhibitor)vs response (three parameters) -...
19 October 2023 2,924 4 View
Our recombinant protein fused to ABD (albumin binding domain) degraded before and after purification (imax, ABD-agarose) into 2 parts. Protease inhibitors didn’t help. We use linker (G4S)3.
19 October 2023 4,546 2 View
Hi All, I am trying to design a high fidelity sequencing experiment using Duplex Sequencing. I understand that Illumina genomic input is usually single cell / clonal dna, but I was wondering...
19 October 2023 8,875 0 View
Hi every body I want to precipitated 2 proteins that have interaction in the HEK293T cell. The candidate protein is RIPK1, the kind of protein kinase. How can I do the CO-immunoprecipitation...
18 October 2023 5,737 0 View
Hello, I've been doing protein purification more in the lab and I want a better understanding of the technique. I've been looking into it on my own but was wondering if anyone knew of a good...
18 October 2023 1,397 3 View
I was looking into developed a sustainable Human gut microbiome in-vitro. If you have gone through any pubished paper or have any idea of developing gut microbiome in-vitro, will be appriciated...
18 October 2023 1,352 0 View
How does population growth affect economic development and environment and relationship between economic growth and poverty?
18 October 2023 3,721 0 View
I would be very grateful to learn about current projects in this area of research. Thank you very much Harald Klingemann
18 October 2023 4,536 0 View
I was just thinking could it be possible just because 2- 5 hr storage of my yeast cells before soptting could switch to anaerobic or post diauxic shift
17 October 2023 4,941 1 View
What are the most important applications of complex differential equations??? Are there complex differential equations with fractional orders??? and what are its applications???
17 October 2023 2,120 1 View
Hi all, I am trying to extract RNA from snap frozen leaf tissue ~100-150mg. I am getting good yields >150ng/uL normally with 260/280 >2.1 and 260/280 >2 as well. This is a good sign,...
17 October 2023 4,663 3 View
I want to extract acetic acid from orage as I want to synthesize paracetic acid using acetic acid and hydrogen peroxide
16 October 2023 1,774 1 View
I make a concentration of 50 ng/mL VEGF in endothelial cell growth medium. I add this medium to my microfluidic device to stimulate HUVECs for angiogenesis. I was wondering how long I can use...
16 October 2023 4,826 1 View
How do you make an organic farming project? Its Components.
15 October 2023 8,184 2 View
I am running an experiment on phytoremediation where I am using polluted river water for remediation. During the first experiment there was severe algal growth on the control pots (pots without...
15 October 2023 614 2 View