Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
2,990 views 5,889 posts
Questions related to Plasmids
What role does statistical analysis play in optimizing traffic flow in urban transportation networks?
2 weeks ago 6,852 1 View
If you allow generative AI in your classroom, what kind of assignment or exam still gives you authentic evidence of individual student learning—and how do you grade it?
2 weeks ago 5,639 8 View
Dear everyone, We found in the experiment that the action of a peptide is opposite to that of its known receptor, indicating the existence of a new receptor. How to determine the novel receptor of...
2 weeks ago 2,919 1 View
Mal-PEG4-NHS ester The experimental goal is to conjugate an IgM antibody (total molecular weight 144.7 kDa) to a single-stranded DNA (20-30 kDa), which has a thiol group at one end, using this...
3 weeks ago 8,679 1 View
PEI Transfection Reagent (HY-K2014, MCE) Before plasmid transfection, do 293T cells need to be cultured in serum-free medium for 1 hour before adding the transfection reagent-nucleic acid...
3 weeks ago 574 2 View
How does Fourier analysis contribute to the development of digital communication systems, particularly in modulation schemes?
4 weeks ago 8,243 2 View
How can Fourier analysis be integrated with machine learning to improve feature extraction for pattern recognition?
4 weeks ago 6,554 3 View
artículos que hablen sobre la formulación de repelentes de mosquitos
4 weeks ago 4,890 1 View
I have tried to purify Pichia secretory protein of 80kDa. Initially precipitated the soup protein using ammonium sulphate precipitation. Then resuspended the precipitation in 1x PBS ( 20mM...
5 weeks ago 3,873 2 View
Hi everyone, I’m trying to extract DNA from a bacterial community culture using the FastDNA SPIN Kit for Soil. The extractions give me DNA, but I keep running into a problem: there’s a lot of salt...
last month 6,409 3 View
How exactly they work
last month 6,761 3 View
My question is whether I can infiltrate Cy3-labelled ssDNA or siRNA into intact plant leaves without carriers (agrobacterium or gene gun) and still have a chance to see fluorescence through a...
last month 7,800 0 View
Does anyone have any experience regarding the estimation of ATP in muscle (meat) using a bioluminescence assay?
last month 5,758 0 View
When performing CCK-8 assays on the HCC95 cell line (concentration gradient 0–20 mM), no obvious dose-response trend was observed. Are there any literature reports supporting the use of...
last month 2,324 1 View
Hello, I am working on bacterial enzyme-mediated degradation and am particularly interested in cytochrome P450 enzymes. If you have experience with expression, functional assays, I would greatly...
last month 2,818 1 View
I am studying pMLKL Translocation to plasma membrane. I am using Tx-114 system for isolating plasma membrane protein from HT29 cell. I have used NaK ATPase as a loading control for the western...
last month 2,412 0 View
I would like to know what errors during the process of gene cloning can potentially lead to genetic instability, as it often relies on the precise insertion of a target gene into a suitable...
last month 1,021 1 View
Hello, I am working on circular RNA isolation and applying RNase R treatment. Although my total RNA quality is within the standard range before the reaction, I observe a significant decrease in...
last month 4,886 2 View
Hello friends. I have an isocyanate prepolymer that is white in color and heterogeneous (containing solid particles) with high viscosity (gel-like). This prepolymer is stable in a closed...
last month 9,684 3 View
Recently, I purified DnaA proteins from two different species via nickel-affinity chromatography. Based on their respective theoretical pI values, I prepared tailored purification buffers....
last month 3,407 4 View
I am trying to analyse confocal images of coronal brain sections to compare non-injected (contra) vs injected (Ipsi) hemispheres of control (pbs) animals and treated (agent) animals. The...
08 August 2025 6,558 1 View
Hi All, I have tried many times to probe for E-Cadherin in Western blot but I am unsuccessful. I am using sheep tissue (mainly aorta right now) and I use a RIPA Extraction buffer. Does anyone have...
05 August 2025 4,769 3 View
Hi everyone! I'm having trouble with my Internal Control in a real-time PCR assay. The kit protocol says to add the IC "directly to the sample/lysis mixture" during DNA extraction, so I'm...
01 August 2025 1,591 1 View
I followed this protocol but did not see a band, but instead of Gel Loading Buffer I used a loading buffer that also contained GelRed and did not pre-heat the gel to 50°C: Prepare a 15% denaturing...
31 July 2025 5,790 1 View