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Questions related to Peptides
I downloaded a PDB structure of a protein and the FASTA sequence of the same protein. When I imported the structure in PyMol, the sequence represented in one subunit of a protein is only a portion...
02 July 2020 5,987 8 View
Hello, I will be doing protein interaction pull down with the aforementioned kit. I will be using 50ug/100ul of biotinylated peptide as bait. I am wondering how much of the lysate (amount and...
18 June 2020 6,305 0 View
Hi, I am looking for a better software to analyse post translational modifications on the proteins after Mass Spectrometry. I am purifying AMPylated (Adenylylated) peptides through a two step...
03 June 2020 1,014 4 View
We would like to couple peptides or proteins to Heparin (we have a Heparin solution from Leo Pharma). Is there a simple way of doing this, for example with some sort of crosslinker one can buy...
29 May 2020 2,172 0 View
Hello, Cytiva has discontinued Thiopropyl Sepharose 6B , product # 17042001. Would anyone be able to suggest alternatives to this product? Thanks in advance.
28 May 2020 5,203 2 View
Hi Researchers, I'm a PhD student in molecular biology and would really appreciate some assistance with interpreting data related to high-res mass spec. I understand this is possibly a bit...
27 May 2020 6,564 0 View
Is there a way other to separate a peptide with molecular weight smaller than10kDa by electrophoresis Except for tris-tricine sds-page?
26 May 2020 9,739 6 View
how we can easily understand about blue shift and redshift in Raman spectroscopy, is anyone able to help?
26 May 2020 7,399 6 View
Please elaborate with some examples and any valid references if one has.
22 May 2020 7,390 3 View
Hello everyone, Our group recently performed MS/MS spectrometry to study the possible alterations of a stimulus in specific protein phosphorylation. After obtaining the results we in fact...
11 May 2020 2,702 0 View
Dear all, I am studying the role of antihypertensive peptides (AHPs) in which the efficacy of tripeptide Leu-Lys-Pro (LKP) will be assessed. Until now, in vivo animal studies were done in...
06 May 2020 9,113 0 View
Hi. I need to make vertical device cause my crystal has too big resistivity to make it into lateral form. Unfortunately, our lab doesn't have a micro-manipulator so i have to use only...
06 May 2020 2,040 0 View
ACE2 serves as the entry point for SARS-CoV2 since it is a transmembrane protein. When SARS-CoV spike S1 protein binds to ACE2 enzymatic domain, located on the surface the cell, leads entry of the...
01 May 2020 5,138 3 View
Can anyone suggest me any software/webserver to predict the ADME/Tox properties of peptides?
29 April 2020 952 1 View
I tried Mild Acid Elution to isolate MHC-I bound peptides from the surface of mouse melanoma cells. Therefore, I harvested 1,6 x10^8 cells, pelleted them and washed them three times with PBS....
27 April 2020 7,271 0 View
I use a Qiagen TissueLyser II to extract total RNA and DNA from skin tissue biopsies for microbial analysis. I'm just confused on the use of beads, I notice some people use larger 5mm stainless...
23 April 2020 8,947 0 View
I'm curious of the prevalence of Phe-terminal peptides and proteins in the human body. I haven't been able to find a source that gives an indication of this. The ones that come to mind for me are...
31 March 2020 3,224 2 View
Dear all, Hi, This question probably is similar to the question that some scholars have already asked; but they have generally been about the popular distros of researchers. It is noteworthy that...
30 March 2020 811 5 View
I am testing different combinations of peptides grafted to alginate hydrogels. Inside these hydrogels I have culture human mesenchymal stem cells and now I want to see how the different...
25 March 2020 5,659 4 View
Generally I came across for small organic molecules like doxorubicin localization in pluronic micelles but not for larger molecules like peptides.
12 March 2020 5,587 3 View
Dear collegues, I am recently struggling with the dot blots I am using for quantification of peptide conjugated to polymer nanoparticles. I have this fluorescently labelled peptide, which is...
04 March 2020 9,984 2 View
Greetings, Does anyone have information about the shelf life of the following cocktails" Protease Inhibitor Cocktail Set-III (Calbiochem) Phosphatase inhibitor cocktails Set-II...
28 February 2020 4,407 4 View
I am presently working on modification of carrier vehicles to be used for physiological systems. Peptide conjugation is required for this to achieve a targeted delivery system. Kindly suggest some...
23 February 2020 6,077 3 View
I am trying to understand the nature of a H2O2 neutral loss in HCD fragmentation data of cysteic acid containing peptides. The y-series showing the neutral loss is complete so we believe it must...
14 February 2020 6,038 2 View