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Questions related to Peptides
Hi, I am trying to determine 4beta-hydroxycholesterol in plasma. It is endogenous, for this reason I need to use a surrogate matrix (1% BSA) but I have problems with this protein. 1. In the first...
05 May 2023 330 4 View
Open Journal of Clinical and Medical Case Reports (ISSN 2379-1039) (jclinmedcasereports.com) Not indexed on PubMed but NLM/PubMed ID: 101658769 (citations only) It claims Impact Factor:...
04 May 2023 986 1 View
I tried to detect the endogenous expression of my protein of interest, but I can't. However, at the same time, I can verify the overexpression of the protein with different antibodies. I tried...
03 May 2023 3,648 4 View
In stale seed bed technique, before sowing the field was irrigated and favoured for germination of weeds after few days the nonselective herbicide was sprayed to kill the weeds then crop was sowed...
02 May 2023 9,058 2 View
What is the influence of the Himalayas on Indian agriculture fertile soil and influence of the Himalayas on Indian agriculture and climate?
01 May 2023 7,795 4 View
How to understand fully crossed and randomized within-subjects design?
01 May 2023 4,203 2 View
We are using Mobitech bacillus system but that is not working
28 April 2023 2,702 0 View
I performed crosslinking using a 10 mg pellet and 5 ml of diluted 2.5% glutaraldehyde for a period of 2 hours. Upon attempting to dissolve the crosslinked enzyme for activity assay, I encountered...
28 April 2023 6,103 3 View
Please describe a method preferably where the sample can be used in dry form.
28 April 2023 7,427 2 View
What is the influence of Himalayas on Indian climate and agriculture and importance of Himalayas in promoting agriculture?
28 April 2023 1,855 2 View
Hey everybody, I am looking for an easy method and also software for analyzing TCR repertoires. is Spectratyping the easiest method? do you suggest any method which is easy to set it up in the...
25 April 2023 9,930 4 View
Anatomy and movement assignment 1 sub question
24 April 2023 7,045 3 View
why do i get asterisks in gridpoint 2? i am new to this ONIOM topic. it is a single point energy calculation, where in the high layer there are 18 residues and a ligand, and in the low layer the...
24 April 2023 8,464 0 View
Can a drug show cytotoxicity at 24 hours time point but cannot show cytotoxicity at 48 hours in MTT assay? Or there is a possibility that somewhere the experiment has gone wrong? The mode of...
20 April 2023 4,484 1 View
Although many studies have been conducted on the immunogenicity of intranasal acellular pertussis vaccines in mice, I didn't find any studies in human clinical trials. Why haven't these studies...
20 April 2023 9,268 1 View
How do I measure activity of an enzyme if it catalyses a two-step reaction? The final product is what I want.
19 April 2023 379 7 View
I do not sure whether FⅨa is one of the Serine Protease Family. I found the date said FⅩa and FXIa are belong to Serine Protease. My question is expect the spectrozyme fⅨa that cansed as a...
19 April 2023 1,671 1 View
The lower concentration that i did was 1 ppm and the latter was 10 ppm.
17 April 2023 5,514 1 View
For protein extraction i found a protocol that separate phosvitin from egg yolk using ultrafiltration trough 10 MWCO then uses the filtrate. But phosvitin molecular weight is 35 KDa. So how it is...
17 April 2023 4,395 2 View
In the context of my master's project I'm searching for good drugs candidate that can have an neutralizing effect over SVMPs. I've searched in the literature and I found doxycycline acts as zinc...
17 April 2023 3,825 2 View
I want to design a drug delivery system using peptides as targeting ligands which are supposed to interact with cell surface receptors. Has anyone experienced a similar experiment to help with the...
16 April 2023 6,939 0 View
how to formulate it?
15 April 2023 9,301 1 View
For example, are there any risks to soil health or other environmental concerns that should be considered when choosing a weed management approach? How can farmers balance the need for effective...
13 April 2023 4,562 0 View
translatome refers to the entirety of mRNAs associated with ribosomes for protein synthesis Dose that mean it will take only one specific mRNA that is expressed under spesific condition in...
13 April 2023 6,058 1 View