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Questions related to Peptides
I want to conjugate "Thiol-Modified ssDNA oligos" with "Maleimide-functionalized Magnetic beads". The oligos are already lyophilized, and I am afraid the oligos in the buffer may react together...
22 September 2021 3,118 4 View
Hello there, I carried out Protein-Protein docking using the Cluspro server, the problem is that Cluspro provides resulting models as one combined PDB, so I am asking I there is a way I can...
15 September 2021 8,061 2 View
Hello everyone, I'm using Amira for my thesis. I would like to measure some parameters on a 3d model of skulls. I think I understood what Amira can do and I know the basics now. I also followed...
17 August 2021 9,108 1 View
International Conference of Harmonization (ICH) guideline M7 recommends using two complementary QSAR models, expert-based and statistical-based models, for Genotoxicity prediction. I'd be thankful...
13 August 2021 4,323 2 View
Hello I purified the insoluble fraction of a protein expressed in e.coli BL21 using buffers containing 8M urea, what is the best way to remove the urea while keeping the protein soluble? thanks
10 August 2021 3,079 3 View
Hello, I've recently encountered an issue with a pre-mixed PCR buffer containing cy5 as a reference dye. It seems that cy5 signal is completely absent, while other dyes (FAM etc.) are working...
23 July 2021 709 0 View
Hello, I am trying to make an emulsion of CFA and Peptide using a sonicator. Does anyone have a protocol that they can share with me. Thank you.
08 July 2021 5,226 4 View
I got SASA for three complex and apo protein from MD simulation data. Two complexes showed greater SASA value as compared to apo protein and one complex. What can be the reason behind this? The...
20 June 2021 7,807 3 View
I have novel peptide sequences of 20-30 AA in length. I need to dock it with antigenic proteins. I have to do blind docking of the protein and peptide. I used Cluspro for which I predicted the...
09 June 2021 4,252 3 View
How should I run MD on a protein with a unusual amino acid (FGL or formyl glycine)? I would appreciate it if anyone can help to solve it.
07 June 2021 9,986 3 View
I produced recombinant LL-37 and I want to try antibacterial activity of this product. However I cant measure antibacterial of recombinant LL-37, but purchased synthetic LL-37 have expected...
02 June 2021 3,815 3 View
Hello, I am new to genetic editing and would like to ask en masse to see if anyone would have knowledge. I am studying a single amino acid mutation in a protein (glycine to serine) that is common...
22 May 2021 387 0 View
I am labelling my peptides using click chemistry but end up having brown residual pellet upon desalting (peptide clean-up). This contaminant leads to artifactually high nanodrop reading and...
18 May 2021 7,696 2 View
Hello I am just wondering if you have ideas or if you can share review articles or literatures about insights/strategies/methods for structure-activity relationship study of peptides? I am only...
09 May 2021 4,047 3 View
Dear All, I am trying to run an NVE simulation using Gromacs but not able to conserve the total energy for the system. I have gone through the discussion given about the NVE parameters and I used...
30 April 2021 8,627 2 View
I have the forward sequence and reverse sequence of one sepecies that I want to merge them together in ARB software , what should I do ?
28 April 2021 8,284 0 View
I'm trying to make a co-culture with AGS cells and B cells, and we want to do a sorting. Does anyone here know a good surface marker? this cell line doesn't express catenin beta 1, e-cadherin and...
10 April 2021 4,300 4 View
I have collected 6h to 96h drug release data for unilamellar liposomal vesicle by using spectrophotometer. According to data, we have encapsulated chemotherapeutic agent in 80% efficiency. But...
10 April 2021 488 8 View
Hello everyone, What tools should I use to determine the effects of water molecules on ligand binding sites for protein-ligand interactions? (Especially in MD simulations for ligand interactions...
29 March 2021 5,430 1 View
There are examples of small molecules that have intra cellular targets in Gram positive bacteria but very little information about targeting the outer Peptidoglycan layer. Are there any well...
22 March 2021 7,239 5 View
I am currently doing a project on phytochemicals and its anti-cancer properties. My question is actually in two parts: I have a ligand molecule ( a dietary phytochemical) and I am studying its...
22 March 2021 8,618 5 View
Hi researchers! We recently have bought amyloid beta 1-42 peptide from Genscript. According to the material data sheet, the peptide is soluble in water. However, after examining the amino acids,...
19 March 2021 2,477 9 View
I know that SPARTA and ChemShift applications work best for predicting backbone chemical shifts of proteins and peptides. However, it doesn't work if there are multiple chains of the molecule. I...
15 March 2021 8,775 2 View
I need to prepare a bacterial lysate of mycobacterium where liquid culture contains 10^8-10^9 cells per mL. To increase yield I sonicate 15 mL at once. I tried different regimens of sonication,...
10 March 2021 626 6 View