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Questions related to Peptides
Hi all, I was recently trying to calculate the concentration of amyloid beta concentration from the UV-Vis result. I found that it can be calculated based on absorbance at ~280nm with an...
28 June 2023 4,183 1 View
I am determining the in vitro release profile of citral from chitosan nanoparticles. I will be using the dialysis method using PBS at different ph as the release buffer. During the dialysis, once...
27 June 2023 9,274 2 View
Hello all, I am in the process of setting up an industrial enzyme production facility, initially for producing poultry feed enzymes. Does anyone have a contact of where I can procure the strains...
26 June 2023 6,489 1 View
In field experiment what is the use of co effecient of variance?
26 June 2023 5,918 2 View
Recombinant human cysteine protease inhibitor as antiviral agent against HCV cysteine protease
24 June 2023 151 0 View
I'm unsure why the master curve drawn by TTS appears to be discontinuous. Can you provide more information about the issue?
20 June 2023 9,809 0 View
Is it possible that a small protein goes through a nitrocellulose membrane? if the answer is yes, what´d it be the conditions that can be adjusted to prevent such passage.
19 June 2023 6,367 2 View
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14 June 2023 4,940 3 View
My experiment includes synthesis of polymeric conjugate using hyaluronic acid and a flavonoid molecule in DMSO. Followed by purification of the conjugate by using dialysis membrane (MWCO- 12KDa)...
12 June 2023 1,198 3 View
Skills, Values, Attitudes, Characteristics
09 June 2023 1,009 2 View
I am following this protocol (attached) but I get more of side product (bromo hexane) and very less amount of 1-bromo-2-hexanol.
08 June 2023 6,179 0 View
in MTT assay we use DMSO as solvent ,, but for how long? and is it necessary to be kept in dark place ?
06 June 2023 7,552 5 View
Need this for comparison Elisa outcomes. THX
30 May 2023 2,439 0 View
We are planning to use 4-SULFAMYLBUTYRYL NOVASYN TG RESIN (catalog no - 8550440001). Please suggest us if we can use microwave coupling method against this resin or not? Will there be any problem...
27 May 2023 8,691 0 View
Hello all, first post on ResearchGate! I have been performing immunoprecipitations with FLAG beads (abcam, ab270704), eluting using SDS loading buffer (with DTT) and boiling. The eluted products...
23 May 2023 9,060 5 View
Hi everyone, I'm carrying out a peptide coupling reaction, with the coupling reagents being N,N'-Diisopropylcarbodiimide (DIC) & hydroxybenzotriazole (HOBt). I'm making glycine to couple with...
17 May 2023 5,028 1 View
We are in discussions with Agilent to acquire a custom mutation and fusion panel. They introduced us to the pre-capture polling solution. I would like to know about the experience of those who...
15 May 2023 9,485 2 View
I am trying to construct a phylogenetic tree between some protein sequence alignments. I am struggling however with the interpretation of which tree I should use as the program seems to make many...
10 May 2023 5,266 1 View
Hello, I am designing a plasmid with an SV40 promoter-driven antibiotic resistance. Does expression from an SV40 promoter require a TATA box upstream of the transcription start site? The...
09 May 2023 1,780 4 View
I bought indium nitrate hydrate (alfa aesar - purity 99.99%) which is said to be water soluble. However, it does not dissolve well both deionized water and organic solvents, above 20 mM. Could you...
09 May 2023 965 3 View
What is the relationship between ecosystem complexity and resilience and difference between resistance and resilience biodiversity?
06 May 2023 324 2 View
I´m performing a meta-analysis and some studies report continuous data as percentage of change (pre vs. post) but does not report standard deviation from this percentage of change. I´ve been...
05 May 2023 2,331 3 View
Hey all! I have a question about my proteomics data evaluated in Proteome Discoverer. I got three volcano plots of three biological groups in a ratio with control group. In one group I see a...
05 May 2023 6,798 3 View
Hi, I am trying to determine 4beta-hydroxycholesterol in plasma. It is endogenous, for this reason I need to use a surrogate matrix (1% BSA) but I have problems with this protein. 1. In the first...
05 May 2023 287 4 View