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Questions related to Peptides
The previous version with the QUAL plugin, allowed to convert *.raw files to *.cdf. No more!????? Is there any other way to convert to CDF? I need CDF (not mzML or any other version...)....
08 January 2024 1,367 0 View
kindly throw some light in identifying research problems for the cloud computing & security research topics / areas
04 January 2024 211 2 View
Recently i started a proteomics of blood plasma (100 ms/ms files .raw) against fungal fasta (from uniprot) using MaxQuant in linux. I started with 6 .raw then 30 and finally all 100 files. In 6...
03 January 2024 3,200 5 View
I'm running MQ on some peptide-level enrichment TurboID samples, so I'm interested in quantifying individual peptides. In past versions of Maxquant (v1.6.14 etc), the peptides.txt file contained...
03 January 2024 3,189 5 View
Q2. How to make stock of monoclonal antibody concentration if it is 1mg/ml?
02 January 2024 6,633 2 View
DOC stands for Dissolved Organic Carbon and BOD stands for Biological Oxygen Demand.
19 December 2023 6,244 3 View
I am trying to learn RNA quantification from BV2 cell using easy blue reagent. but the RNA quantity is very low. may be the cell density per ml is low. i m using 6well plate . i cant find where i...
15 December 2023 9,489 1 View
I have read a research paper "Development of a novel morphological paclitaxel-loaded PLGA microspheres for effective cancer therapy: in vitro and in vivo evaluations". I want to synthesize PLGA...
14 December 2023 9,913 1 View
I have encountered precipitation issues during the sol-gel synthesis of La2NiO4. Initially, I prepared stock solutions of lanthanum nitrate hexahydrate, nickel nitrate hexahydrate, and citric acid...
13 December 2023 7,237 2 View
I am currently working with PacBio SMRT sequencing data acquired from various sequencer platforms, including Sequel I, Sequel II, and PacBio RS II. These platforms generate both Circular Consensus...
06 December 2023 8,355 0 View
hi, There are many RNA aptamers with 2'-F pyrimidines, such as CTP and UTP. I understand that 2'-F pyrimidine effectively improves serum stability. My question: Do they matter in terms of...
05 December 2023 363 0 View
Is it highly recommended to use northern blot to ensure my RT-PCR results instead of LC-MS for example? Thank you in advance, Kiriakos
04 December 2023 1,846 2 View
Dear all, I'm currently working with Neisseria spp. bacteria and due to a mistake in the growth media, the bacteria is now trapped in a starch pellet after centrifugation (there is an attached...
03 December 2023 2,698 1 View
How can I find "PCR markers" and "suitable antibody to Flow cytometry" of C2C12 cell line? Is there any web server to find this data?
03 December 2023 3,295 0 View
I want to know, when I need to discard my iPS cells, despite they show good differentiation efficiency? What could I see under microscope?
27 November 2023 2,288 2 View
Good greeting Can you help us to get the references (books, thesis, dissertations, ect.) with all fields and legally form, In order to save it at the electronic library of ministry of youth and...
20 November 2023 8,895 1 View
I want to prove 3 transcription factors from a complex to regulated a gene's expression. How can I prove these three proteins interaction.
13 November 2023 5,369 3 View
Hello, I am searching about the antibacterial activity of mersacidin antimicrobial peptide, the majority of papers used of recombinant form of this peptide for investigation of the biological...
08 November 2023 9,695 1 View
What is serial subculture preservation of microbial culture and how long can you keep bacterial cultures in the fridge?
03 November 2023 6,334 2 View
I have this problem for sometime that Microglia cells in these PLL coated wells are dying after sometime cause they don't adhere to the coverglasses, I've tried different strategies and it...
25 October 2023 7,464 3 View
Hello, I am trying to measure the Kd of an enzyme. I used a fluorescence-based kit and I did a dose-response for the substrate. I measured over 2 hours the fluorescence intensity. How am I...
20 October 2023 7,281 1 View
For treatment of water (river), what is the life of ceramic membrane? For example, 5 years or 50 years? MF or UF? Operation of ceramic membrane after usage: should be kept under water like...
17 October 2023 3,561 6 View
Hi, Have you any tips to dissociate a fixed skin tissue and get good cell quality for single cell RNAseq? I want to perfom scRNAseq on fixed skin tissue using 10x genomics protocol. However they...
17 October 2023 7,691 2 View
we are studying the stability of immobilized lipase in oil, could help to get one copy of "DIKS, R.M.M: "Lipase stability in oil" LIPID TECHNOLOGY, January 2002 (2002-01), pages 10-14"? thanks alot.
16 October 2023 6,301 0 View