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Questions related to Peptides
I am trying to covalently attach the RGD peptide sequence to PNIPAM-co-AAc microgels already coated on different substrates (Si, Glass and Polyurethane). I am going to use the common carboxylic...
11 April 2023 8,008 0 View
BiFC（bimolecular fluorescence complementation） is a useful method to study protein interaction. But what makes me surprised is that the usage is enriched in plant research field. I have no...
04 April 2023 2,592 2 View
Specifically, I am wondering if I can predict the surface marker expression of CD14 and CD16 on monocytes based on scRNAseq data that includes the expression level of those two genes. Does anyone...
30 March 2023 6,795 3 View
When I performed an energy decomposition per residue analysis, it was observed that ligand exhibited a significant energy contribution value when compared to the amino acid residue. In fact the...
28 March 2023 6,864 0 View
Hi, I am performing MTT assay to measure cell viability of ovarian cancer cell line (A2780) upon treatment with Cisplatin. However, I am repeatedly getting the following results and not sure why I...
28 March 2023 8,329 2 View
When we are in the step of aligning virulent factors against human proteom to exclude those proteins with > 35% homology what is the output that we have to use for the next step of predicting...
25 March 2023 3,120 2 View
Hi all, I am currently having many issues processing DDA .raw files with Skyline. DDA were acquired using Xcalibur 4.2.47 and a Thermo LTQ XL. With the previous version of Xcalibur installed it...
24 March 2023 5,436 0 View
Hi all, I would like to improve IgG denaturation in my sample before running an SDS-PAGE. I have seen papers where 4 to 8M Urea is added in the sample. Can anyone tell me if this concentration...
20 March 2023 9,099 10 View
DAR: 1.6 VS DAR 4.0
20 March 2023 3,778 3 View
I'm looking for researchers who widely use flow cytometry assays in rats, specifically panels for T, NK, b cells and their subpopulations. I'd love to know which one of the companies is...
11 March 2023 5,952 2 View
I am optimising the TransIT LT1 reagent for CHO-DP12 cells . I have only achieved an efficacy of 6%. Does anyone have experience with this cell line and reagent and what protocol and efficacy did...
09 March 2023 2,967 1 View
Molecular Docking Using MOE 2016
05 March 2023 8,294 3 View
I want to reduce the interaction between my biotinylated peptides with the plastic and blocking with BSA is not helping.
03 March 2023 4,826 2 View
Hi! I use UV and photoinitiator to gel my PEG-acrylate hydrogel. But I observe that when the temperature is lower (at night during winter), it's very easy to get pre-gelled before molding, even...
02 March 2023 9,835 1 View
Let me know if you have any experience in this subject or any interest. Also I need some articles about this field and authors who worked in this field.
27 February 2023 9,253 4 View
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27 February 2023 1,577 1 View
Previous attempts of various methods such as organic solvent precipitation, ultrafiltration, and recently published in the journal of proteome research, a new method -SPD (sequential precipitation...
25 February 2023 8,430 0 View
I have an LTQ Velos Pro LQIT on which the ion gauge just burnt out. However, Thermo is currently backordered on this part and I am in need of this instrument before it will arrive from them. Does...
23 February 2023 5,620 1 View
Catalase in 440 nm
21 February 2023 3,972 2 View
How can I use 1 methylcyclopropene and ethephon in callus culture? Any idea about the concentration and procedures?
20 February 2023 9,540 0 View
I want to do bioluminescent imaging in tumor-bearing mice. Can I use the ChemiDoc MP system(BR)? There is only an article I found (doi: 10.1016/j.omtn.2019.01.007). Thanks.
18 February 2023 4,126 0 View
In one of Chemical science paper, they mentioned just get the absorbance at 494 nm. Can i get the nanodrop reading at 494 nm for FITC labelled peptide? How accurate is this? Plz suggest.
16 February 2023 9,614 3 View
I prepared a certain probiotic supernatant each of (10x9 cfu/ml). According to literature, i need to prepare a serial dilution of this supernatant in mg/ml or Mg/ml to start MTT assay. How can...
14 February 2023 7,882 1 View
I have extracted DNA from desert soil using a commercial Qiagen kit. When I check my DNA on Qubit and Nanodrop I consistently get very low readings (
13 February 2023 8,270 3 View