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Questions related to Peptides
Hi I want to make a recombinant m13 phage displaying my peptide as a fusion peptide on its surface pVIII. Should i add ATG at the beginning when i want to insert it in phagmid vector? Previously,...
19 December 2020 10,093 2 View
Proteins generally absorb UV light at 280 nm while peptide bonds absorb UV light at 214 nm. When quantifying proteins using the Lowry and Buiret methods, absorbance or optical density is measured...
17 December 2020 1,237 3 View
I'd need a program able to perform dockings with small peptides, similar to Swissdock (so which gives me fullfitness values and delta G values for each cluster calculated). I already tried with...
08 December 2020 2,881 3 View
Can anyone suggest antibody (suitable for WB, IF) against catalytic domain of human DNMT3A (amino acids P602-V912)? I went through several antibodies, but they were generated using synthetic...
04 December 2020 8,758 2 View
I am designing a small peptide to inhibit a homo-dimer of a small secretory protein (about 20 KDa). I need suggestions on, which option would be better? a straight-chain peptide or a ring-shaped...
04 December 2020 4,267 5 View
Hey! I need help on peptide (22 aa) oxidation.This peptide has no methionine, 1 tryptophan & 1 tyrosine. also has 4 cysteine so I used 2 protection groups (trt & acm). For ACM removal, I...
04 December 2020 3,355 2 View
Hi, everyone: I want to determine whether IL-2 and IL-15 have different effects on immunized CD8+ T cell effector function. In this study, I immunize WT mice with peptide on day 0 and 7, and...
03 December 2020 9,553 3 View
I have extracted protease from culture filtrate of Fusarium isolates (n=14) grown in PDB media. For partial characterization, I checked activity at different pH, temperature and in presence of...
02 December 2020 3,460 10 View
Hi, I have been looking around for ways to insert various monomers of an AMP in membrane bilayers. To start-off, I have been using Charm-Gui, but it seems that I can only insert one peptide at a...
30 November 2020 8,751 2 View
Greetings, I have a short stretch of 20 aa with strong binding affinity for a compound. I am trying to de some whole cell flourescence or visible biosensing technique based on this peptide. Can...
27 November 2020 8,444 3 View
Can someone please provide me with a CIF file (crystallographic information file) of a cubic spinel (AB2O4) with a space group I23 (197) ? i need it to confirm a new phase Thanks
26 November 2020 7,327 0 View
Hello! I am working with a peptide of 19 amino acid composition. It has 7 positive charged residues (Arg + Lys) and no negative charged residues so it's obvious the peptide is basic. I tried...
17 November 2020 1,709 7 View
I need to immobilize 3 peptides: CGPDGRDGRDGRDGR, CRQILIWFQNRRMKWKKYQRLC, penetratin peptide RQIKIWFQNRRMKWKKGG on the SiO2-covered gold nanoparticles. What is the most efficient and stable way...
17 November 2020 3,008 3 View
Though many reports says all parts of the plant contains L-DOPA when I had estimated the leaf alone it shows no presence of L-DOPA and same with the callus developed from the leaves. Any idea how...
17 November 2020 7,327 3 View
Dear all, I would like to characterize the potential structure of a insoluble aggregate in biorelevant media. I was wondering if I could do this with circular dichroism and how to do it. Do you...
07 November 2020 4,436 3 View
During a chemical synthesis of peptides by the F-moc method, the following happened: all the initial steps of the synthesis occurred as planned, but in the cleavage, TFA is used. In the lab, there...
03 November 2020 3,142 8 View
Hi all, I am interested in peptide structure prediction services, both secondary structure and tertiary structure. Protein prediction services that can predict structures of short peptides are...
31 October 2020 4,459 4 View
Hey my dear freinds, I wonder how to modify the known antibacterial peptides’ amino acids composition to make them more easily bond to both gram- positive and negative bacteria, which...
30 October 2020 3,730 6 View
Hi everyone, I would like to ask, in PBMC when pulsed by peptide, what are the cells that present the peptide to the T cells? Is it B cell or monocyte? And is it memory T cells that are activated...
28 October 2020 7,062 3 View
Dear researchers : y-randomization is one of the special tests of machine learning approaches. I want to evaluate my developed machine learning-based models, to predict active peptides, by...
27 October 2020 1,433 2 View
In the use of Polyelectrolyte multilayer membranes (PEMM) for the treatment of waste water, different polyelectrolyte systems are considered in the production of the PEMM, most popularly used is...
26 October 2020 9,573 0 View
I found a peptide (15 amino acid) drug which has no definite secondary structure that binds very well to a receptor. I am having doubts regarding it. Is it necessary for peptide drugs to have...
22 October 2020 7,270 4 View
Dear all, I am working on small peptides project. We have two peptides with molecular weights around 6000. These two peptides were soluble in water. Objective is to validate the bioanalytical...
16 October 2020 7,937 2 View
Hi, new member here... I am currently having difficulty dissolving HBTU in DMF to create a .2M solution. I also add NMM and oxymapure but those substances all seem to disolve except for the HBTU,...
09 October 2020 454 3 View