Estimate Beta is .00, yet it's significant?

Hi everyone, I've run an analysis of a latent 3-way interaction in Mplus (Y on XWZ). The estimate for this path is 0.000. S.E. is 0.000. Est./S.E. is 2.034. Two tailed p-value is 0.042. So,...

08 December 2020 2,132 4 View

What is the double layer capacitance of a 1 sq-cm smooth gold electrode in 0.1N (0.05M) Sulfuric Acid?

Is there an empirical or theoretical standard value for this (at room temperature) and for different electrolyte concentrations? Is there a resource available that provides a chart of values for...

29 November 2020 6,937 2 View

How does the concentration of atmospheric oxygen near ground level vary with time?

Hello, I am interested in how the concentration of O2 varies over time close to ground level. I have been informed that the concentration can drop when an inversion layer is present in the local...

19 November 2020 2,328 3 View

How does one calculate the best volume of nickel gel to use in a protein purification?

We know the capacity of the gel and the total amount of protein in the bacterial lysate from a Bradford assay. What we do not know is what fraction of the total protein is the his-tagged protein...

15 November 2020 4,664 2 View

Final temperature of water leaving an ice bath through copper tubing?

I have water flowing at 4 GPM at 2.5 feet per second through 50ft of 0.375 in. outer diameter tubing with 0.014 in. wall thickness. The water flows through copper tubing into the 50ft coil...

01 November 2020 8,039 5 View

Is it acceptable to publish data that were digitized from a previously published plot/figure (referencing the original figure of course)?

There are many ways to extract original source data from figures and plots in publications. If one were to do that and reanalyze the data in the same or a different way, is that acceptable in a...

28 October 2020 3,822 2 View

Methods to attach glutathione agarose resin to glass slide?

Hello, I am looking to capture a GST tagged protein on a glass slide. I was wondering if anyone had any experiences with glutathoine agarose resin. I realize this is commonly used in affinity...

13 July 2020 1,773 3 View

Where to find predator:prey mass data (for terrestrial carnivores)?

I'm looking for datasets (or databases) in which the body mass of predator and prey are presented. I'm specifically interested in terrestrial predators (snakes, mammals, birds). An example of is...

16 June 2020 2,453 9 View

How to calculate the overlap between animal distributions (Shape or .shp file)?

I would like to calculate the overlap between the distributions of two sets of data (predator & prey). The prey set has got about 250 species, the predator set approximately 5000. For each...

21 May 2020 6,726 3 View

Who can assist with an ingredient design of a Covid 19 Sars-2 Cov protein subunit and adjuvant vaccine?

18 May 2020 1,675 13 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View