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Questions related to Peptides
Other than using a positive control, is there a way to determine if the Genscript anti-his elisa protein quantification kit underestimated the protein concentration? Because the discrepancy using...
08 October 2020 5,313 2 View
Hello forum, I would like to know if TBA.OH is volatile in mixtures of acetonitrile or methanol. I want to analyze peptides that were previously dissolved in TBA.OH-conatining solution, but dried...
06 October 2020 7,028 3 View
I immobilize fluorescent peptide on chitosan films and when I try to measure the thickness of the film with ellipsometry, the software says that I have a film with 2 nm or less. However, with...
02 October 2020 3,805 3 View
I want to look at the interaction between fibronectin and integrins on cells in their native conformation. My cells are T24 and MB49 which are adherent cells. Is there a way to lift or suspend...
01 October 2020 6,021 3 View
I would be grateful if someone would advise or give a link on a detailed fluorescence titration technique. I am interested in setting up an experiment on binding of a receptor to a...
29 September 2020 5,791 7 View
Dear everyone, I synthesize peptides with a common Fmoc-strategy starting from a Cysteamine 2-chlorotrityl resin. That works fine and usually I cleave the peptide from the resin with a mixture...
25 September 2020 7,948 2 View
I work with derivatized neurotransmitter molecules. I was using C-18 SPE cartridges previously for desalting them before LC-MS, but now I would like to adopt in-house made stagetips. These are a...
23 September 2020 5,005 6 View
Most concentration filters I have seen available have the smallest cut-off of 3 kDa. What is the best way to concentrate a short peptide with MW between 2 and 3 kDa? Looking for a method, which in...
17 September 2020 9,744 3 View
I have mostly done computational works and the transition to experimental work is slightly demotivating as I am stuck at each stage starting from whether to wear gloves for certain things to why...
16 September 2020 9,579 5 View
I have prepared a fmoc amino acid based peptide having both the SH groups are protected with triphenylmethyl (i.e, trityl) group. Actually I want to do mono deprotection. I have a little compound...
02 September 2020 5,018 6 View
Hello, I was wondering if someone has ever tried to use the BioID technique to identify extracellular interactions such as ligand/receptor interaction? Thank you in advance
28 August 2020 8,125 2 View
Dear researchers I can not find the papers that clarify the role of hydrophobic residues including Ala, Leu, Ile, Val, Trp, and Phe in the anticancer peptides( ACPs) activity and in the...
22 August 2020 3,591 5 View
Dear friends, I want to design a short length peptide (eg. 10aa) for coating at the ELISA wells. Then I want to use the serum of patients as Ab1 then the HRP-labeled anti-human IgG as Ab2. I don’t...
16 August 2020 9,427 2 View
Let's take an example like my lipid-based formulation showed ~ 110mV zeta potential, I am a bit worried about the stability of formulation after keeping it in storage for a few months. Thanks in...
09 August 2020 6,778 7 View
Hello everyone, I would like to use a biotinylated 5AA peptide to perform a Pull-down/MS experiment, to screening peptide-binding receptor proteins. But I'm not sure if this experiment will work,...
04 August 2020 243 2 View
I am doing molecular docking using cDocker in discovery studio. But, I am confised how tointrepret the docking results. Which value is more important CDOCKER ENERGY or CDOCKER_INTERACTION ENERGRY?...
03 August 2020 5,941 4 View
Hello, I'm entering a project which delves into proteomics (woo). At one point in the protocol, we evaporate samples under Nitrogen. Is it possible to store this sample after the evaporation step...
02 August 2020 8,545 3 View
I am trying to improve animals mineral-mixture feed supplement at commercial scale. For this purpose, I came to know that there should be chelated compounds instead of simple one. Like CuSO4 or...
20 July 2020 7,793 0 View
Hi everyone, I wonder if there is a general guidelines for selection of the right peptides in PRM-LC-MS/MS. Many thanks in advance for your response(s).
18 July 2020 8,144 4 View
I have a sample with a volume of less than 10 mL, and a fairly low concentration of 24 mg. I want to do a peptide cut-off, but i'm worried that my cut-off results will get poor results. What can i...
17 July 2020 8,450 7 View
I have a question for HPLC and LC/MS experts. I'm trying to develop a LC/MS (SIM mode) method for separation of coeluting impurities of synthetic peptide (double addition impurities). While most...
15 July 2020 1,693 1 View
I am incubating bacterial vesicles that contain proteases with human proteins. I want to identify the site of cleavage in these proteins but I would like first to separate the vesicles from 1)...
09 July 2020 5,740 1 View
I'm analyzing recombination protein samples that contain a lot of predictable PTMs. Using MaxQuant output tables: From either the ProteinGroups table or Petides table I can obtain the intensity...
03 July 2020 383 1 View
Dear researchers, I need to draw relatively long peptide (40 residues) containing a non-amino acid linker as well as some organic radicals like fatty acid parts and others. On the next step I...
03 July 2020 2,366 0 View