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Questions related to Peptides
I am extracting protéines by isoelectrique precipitation at pH 4. I don't know if I have to resolubilise the proteins at pH7 before freez-drying or I can freez-dry them directly after...
11 February 2023 8,854 4 View
Hi, I am running a test docking for 3DTC protein and Vin inhibitor (both taken from PDB). I think I have taken the correct steps before running both Autdock and Autogrid. I do not encounter any...
10 February 2023 3,769 3 View
Hi all, I am about to perform Lipofecatmine 2000 kit-based transfection in SHSY5Y (mammalian) cells. I have some questions regarding the minute details in the protocol, which I hope you can help...
04 February 2023 173 6 View
Hi! I have a peptide which has nonstandard amino acid NAL at the N-terminal and C-terminal, and I want to use CHARMM36 force field to generate it’s parameters, there are no dihedral parameters...
03 February 2023 3,293 0 View
I would like to know importants, challenges and it's negative effect.
28 January 2023 3,982 0 View
I'm talking to my students (due to necessity) about how it's important that they don't make sexual comments about their female lecturers' appearance. As they're learning psychology I'd like to...
27 January 2023 4,867 4 View
How to find the conductivity of any metal oxide (TiO2, WO3,.. etc.) pellet form?
20 January 2023 1,944 3 View
Realism has been the back one of physics metathinking i. E insistence of a truth marker for every truth. It has a big influence in deciding how to incorporate or reject new ideas about progress...
20 January 2023 9,829 2 View
Dear all friends of research gate, I want to understand how to calculate the sheet resistance theoretically for the developed layer stack design on a glass substrate for my research paper....
20 January 2023 1,797 4 View
we have synthesized 27Mer peptide which contains 8 glycine amino acid. and due to inefficient coupling one of the glycine coupling was partially completed giving us the peak of desglycine peptide....
13 January 2023 1,043 1 View
Dear all, I need to perform isotopic determination of Kr-85 in a gas matrix. Unfortunately, I'm no expert in the field, and I'm struggling to find which Mass spectrometer I should purchase. I...
12 January 2023 6,878 2 View
After completing the required steps to build a machine learning model (Training and testing). How to evaluate the model using new data ?. Assuming we built a classification model with Python using...
11 January 2023 3,230 3 View
Dear colleagues, I am searching for a fish cell line to study the natural extracts' immunostimulant activity. Could you please provide me with information about the best model to use, and how to...
10 January 2023 8,487 4 View
We want to synthesize citrate salt of a peptide and we used ammonium citrate to form a counterion to trifluoroacetate. citrate salt is formed perfectly but after lyophilization the weight gain was...
02 January 2023 5,144 2 View
For western blot sample preparation of mice brain tissue (control and transgenic groups), I used RIPA with stainless steel beads for homogenization. After centrifugation, transgenic samples had a...
27 December 2022 405 3 View
I designed a peptide to inhibit angiogenesis, I want to know if it interacts with itself and forms a complex?
27 December 2022 2,368 4 View
I have done my qPCR experiments and gave me some results, I used the DDCt method and I calculated the 2^(-DDCt), I transformed my data in base 10 logarithm and separated my samples between control...
22 December 2022 5,371 4 View
If I choose amino acids that is important for the antimicrobial activity and modify them to stabilize the peptides, I will decrease their activity ?
22 December 2022 3,512 4 View
Hi, I've been conducting an experiment regarding the antimicrobial activity of my soil bacteria against MDR strains using well diffusion method. I'm performing the experiment using bacterial broth...
21 December 2022 1,000 6 View
I am stuck with a 64 Mbit file containing about 45000 bacterial sequences in fasta format from a NGS-sequencing project with a PacBio RS. The sequences are about 1429 bps long and from a...
21 December 2022 3,657 5 View
I want to prepare a Rose bengal test
17 December 2022 265 0 View
if same bacteria from a colony grows on both macconkey agar and mannitol salt agar research gate
16 December 2022 5,616 2 View
I'm having problem with installing MiniION Software for Mk1b. The program keeps asking to restart the computer. I reboot and ask again. We stay in this looping
14 December 2022 4,625 1 View
SchNetPack is a toolbox for the development and application of deep neural networks to the prediction of potential energy surfaces and other quantum-chemical properties of molecules and materials.
13 December 2022 5,637 3 View