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Questions related to Peptides
For unknown reasons I started to notice that after sometimes (0.5-1.0 hr) of operating crossflow filtration system, the flux increases steadily with corresponding decrease of rejection of the...
31 October 2024 6,204 4 View
Hi, Can anyone suggest me how to get NHEt in the C terminal of peptide during SSPS? I cannot do post synthesis selective modification as the peptide has R and K in the sequence? Should I use any...
23 October 2024 1,819 4 View
I am running a 12 % SDS gel for my protein expression (targets are between 35 - 17 kDa). I use a mini biorad setup for gel preparation. I run at 60V for 30' followed by 100V for 1 hour and 15...
20 October 2024 3,205 4 View
I am preparing to work with Caki-1 and Caki-2 cell line cultures. I've seen a couple of biotech company websites suggesting the inclusion of 2 mM Glutamine to the McCoy's Modified Media that these...
16 October 2024 9,112 6 View
Efficient production of ditiazem using enymes Example: Lipase enzyme Immobilized on glutaraldehyde activated chitosan is used in producing ditiazem chiral intermediate Are there any enzymatic...
10 October 2024 9,419 2 View
Hi everyone, does anyone have experience with the Purpald assay for detecting lipopolysaccharides (LPS)? I would appreciate any insights or tips on optimizing the assay for accurate results....
04 October 2024 201 0 View
Dear colleagues, As a researcher focusing on financial investment and international bank risk analysis, I have encountered some challenges in the European market in my work. I hope to communicate...
04 October 2024 4,278 0 View
what are the advantages of using teak saw dust as a adsorbent for heavy metals over other biomass adsorbent
30 September 2024 604 0 View
I have loaded a hydrophobic drug into silica NPs and have been using indirect approach to measure the loading amount of drug based on the supernatnt but this approach does show me the right...
24 September 2024 3,144 9 View
I have never done any CRISPR experiment before and my tutor asked me to use CRISPR KI to induce a mCherry tag for a gene in U2OS, I decided to follow the protocol described in Generation and...
19 September 2024 7,775 0 View
Hello everyone, I am facing a persistent issue with PCR genotyping of mouse tail DNA for the presence of SFTPC-Cre and G12C genes. Our genotyping panel includes three genes, including p53,...
08 September 2024 6,318 0 View
The expressed cry protein was present in pellet instead of supernatant after sonication. Hence the pellet was dissolved in sodium carbonate buffer with small amount of detergents. I am stuck...
06 September 2024 655 6 View
DETAILS NEED TO BE REQUIRED
13 August 2024 428 2 View
Hello. I purchased a polymer-bound EDC to cross-link, with NHS, a collagen gel. I observed beads on the surface of the gel after the cross-linking phase. I think that they are the polymer linked...
08 August 2024 9,075 0 View
Please identify the effects of adding black soldier fly to laying hen diets on blood profile and antioxidant enzymes CAT and SOD. also please any related studies.
04 August 2024 2,945 0 View
For molecular docking of an organic compound with Bacillus megaterium which protein should choose? Because the organic compund was tested its antimicrobial activity with "Bacillus megaterium" on...
02 August 2024 980 1 View
Understanding that bacteria can live in almost all kinds of environments, but can one specific species of bacteria live in a diverse environments which including as symbiont between plant root and...
05 July 2024 4,448 0 View
When choosing the receptor model (rigid or flexible) in molecular docking, does it depend on the designed ligands' characteristics or anything else? For instance: + With the ligand A, the protein...
26 June 2024 3,179 0 View
General understanding is that postbiotic particularly those that are heat-treated are stable to heat and their activity remains stable during gastric journey. Wondering if this is true for all...
26 June 2024 3,327 3 View
I am doing research in the United Sates America. So, I will pay in US dollar. Could you please tell me how can i get Zirfon membrane? Thank you.
25 June 2024 7,819 0 View
Hey all what are the ways by which an encapsulated drug inside BSA may escape from its encapsulation. We had synthesised curcumin encapsulated BSA by desolvation method followed by centrifugation...
24 June 2024 9,091 5 View
I've been working with activated sludge samples and have encountered a challenge in identifying certain structures/organisms that appear abundantly within the flocs during optical microscopy...
24 June 2024 9,784 1 View
I follow the protocol in this paper "Computational protein–ligand docking and virtual drug screening with the AutoDock suite" for the "Flexible Docking". Everything is smooth until I write the...
23 June 2024 3,470 2 View
Hello, I have paramterize the residue namely “DOP” into both files of amino acids.rtp and lipids.rtp files of GROMOS96 53a6 force field and charmm27… respectively, although I mentioned the residue...
20 June 2024 7,873 0 View