Contact experts in Peptides to get answers
914 views 1,808 posts
Questions related to Peptides
When I performed an energy decomposition per residue analysis, it was observed that ligand exhibited a significant energy contribution value when compared to the amino acid residue. In fact the...
28 March 2023 6,849 0 View
Hi, I am performing MTT assay to measure cell viability of ovarian cancer cell line (A2780) upon treatment with Cisplatin. However, I am repeatedly getting the following results and not sure why I...
28 March 2023 8,319 2 View
When we are in the step of aligning virulent factors against human proteom to exclude those proteins with > 35% homology what is the output that we have to use for the next step of predicting...
25 March 2023 3,110 2 View
Hi all, I would like to improve IgG denaturation in my sample before running an SDS-PAGE. I have seen papers where 4 to 8M Urea is added in the sample. Can anyone tell me if this concentration...
20 March 2023 9,090 10 View
I'm looking for researchers who widely use flow cytometry assays in rats, specifically panels for T, NK, b cells and their subpopulations. I'd love to know which one of the companies is...
11 March 2023 5,942 2 View
I am optimising the TransIT LT1 reagent for CHO-DP12 cells . I have only achieved an efficacy of 6%. Does anyone have experience with this cell line and reagent and what protocol and efficacy did...
09 March 2023 2,954 1 View
Molecular Docking Using MOE 2016
05 March 2023 8,281 3 View
I want to reduce the interaction between my biotinylated peptides with the plastic and blocking with BSA is not helping.
03 March 2023 4,817 2 View
Hi! I use UV and photoinitiator to gel my PEG-acrylate hydrogel. But I observe that when the temperature is lower (at night during winter), it's very easy to get pre-gelled before molding, even...
02 March 2023 9,813 1 View
Previous attempts of various methods such as organic solvent precipitation, ultrafiltration, and recently published in the journal of proteome research, a new method -SPD (sequential precipitation...
25 February 2023 8,421 0 View
I have an LTQ Velos Pro LQIT on which the ion gauge just burnt out. However, Thermo is currently backordered on this part and I am in need of this instrument before it will arrive from them. Does...
23 February 2023 5,609 1 View
Catalase in 440 nm
21 February 2023 3,961 2 View
I want to do bioluminescent imaging in tumor-bearing mice. Can I use the ChemiDoc MP system(BR)? There is only an article I found (doi: 10.1016/j.omtn.2019.01.007). Thanks.
18 February 2023 4,116 0 View
In one of Chemical science paper, they mentioned just get the absorbance at 494 nm. Can i get the nanodrop reading at 494 nm for FITC labelled peptide? How accurate is this? Plz suggest.
16 February 2023 9,601 3 View
I prepared a certain probiotic supernatant each of (10x9 cfu/ml). According to literature, i need to prepare a serial dilution of this supernatant in mg/ml or Mg/ml to start MTT assay. How can...
14 February 2023 7,870 1 View
I have extracted DNA from desert soil using a commercial Qiagen kit. When I check my DNA on Qubit and Nanodrop I consistently get very low readings (
13 February 2023 8,259 3 View
I am extracting protéines by isoelectrique precipitation at pH 4. I don't know if I have to resolubilise the proteins at pH7 before freez-drying or I can freez-dry them directly after...
11 February 2023 8,807 4 View
Hi, I am running a test docking for 3DTC protein and Vin inhibitor (both taken from PDB). I think I have taken the correct steps before running both Autdock and Autogrid. I do not encounter any...
10 February 2023 3,725 3 View
Hi all, I am about to perform Lipofecatmine 2000 kit-based transfection in SHSY5Y (mammalian) cells. I have some questions regarding the minute details in the protocol, which I hope you can help...
04 February 2023 116 6 View
Hi! I have a peptide which has nonstandard amino acid NAL at the N-terminal and C-terminal, and I want to use CHARMM36 force field to generate it’s parameters, there are no dihedral parameters...
03 February 2023 3,248 0 View
I would like to know importants, challenges and it's negative effect.
28 January 2023 3,957 0 View
I'm talking to my students (due to necessity) about how it's important that they don't make sexual comments about their female lecturers' appearance. As they're learning psychology I'd like to...
27 January 2023 4,824 4 View
How to find the conductivity of any metal oxide (TiO2, WO3,.. etc.) pellet form?
20 January 2023 1,906 3 View
we have synthesized 27Mer peptide which contains 8 glycine amino acid. and due to inefficient coupling one of the glycine coupling was partially completed giving us the peak of desglycine peptide....
13 January 2023 1,014 1 View