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Questions related to Peptides
Dear all, I need to perform isotopic determination of Kr-85 in a gas matrix. Unfortunately, I'm no expert in the field, and I'm struggling to find which Mass spectrometer I should purchase. I...
12 January 2023 6,837 2 View
After completing the required steps to build a machine learning model (Training and testing). How to evaluate the model using new data ?. Assuming we built a classification model with Python using...
11 January 2023 3,186 3 View
Dear colleagues, I am searching for a fish cell line to study the natural extracts' immunostimulant activity. Could you please provide me with information about the best model to use, and how to...
10 January 2023 8,441 4 View
We want to synthesize citrate salt of a peptide and we used ammonium citrate to form a counterion to trifluoroacetate. citrate salt is formed perfectly but after lyophilization the weight gain was...
02 January 2023 5,118 2 View
For western blot sample preparation of mice brain tissue (control and transgenic groups), I used RIPA with stainless steel beads for homogenization. After centrifugation, transgenic samples had a...
27 December 2022 380 3 View
I designed a peptide to inhibit angiogenesis, I want to know if it interacts with itself and forms a complex?
27 December 2022 2,331 4 View
I have done my qPCR experiments and gave me some results, I used the DDCt method and I calculated the 2^(-DDCt), I transformed my data in base 10 logarithm and separated my samples between control...
22 December 2022 5,313 4 View
If I choose amino acids that is important for the antimicrobial activity and modify them to stabilize the peptides, I will decrease their activity ?
22 December 2022 3,488 4 View
Hi, I've been conducting an experiment regarding the antimicrobial activity of my soil bacteria against MDR strains using well diffusion method. I'm performing the experiment using bacterial broth...
21 December 2022 952 6 View
I am stuck with a 64 Mbit file containing about 45000 bacterial sequences in fasta format from a NGS-sequencing project with a PacBio RS. The sequences are about 1429 bps long and from a...
21 December 2022 3,587 5 View
if same bacteria from a colony grows on both macconkey agar and mannitol salt agar research gate
16 December 2022 5,577 2 View
SchNetPack is a toolbox for the development and application of deep neural networks to the prediction of potential energy surfaces and other quantum-chemical properties of molecules and materials.
13 December 2022 5,609 3 View
I am doing a test between two dichotomous variables. What does it mean if a McNemar shows a significant difference in the proportion (p
11 December 2022 1,101 6 View
Hello everyone and thanks for reading his question. I found during bacterial protein production that I had set the centrifuge much higher than intended when pelleting intact bacteria. My...
07 December 2022 6,743 1 View
Hello,everyone! I had a problem when I was doing fluorescence spectrum, and I hope someone can give me some advice on how to solve it. I want to explore the interaction between synthetic peptide...
04 December 2022 4,269 3 View
KATSUMATA K, KATSUMATA K, Jr., KATSUMATA Y: Protective effect of diltiazem hydrochloride on the occurrence of alloxan- or streptozotocin-induced diabetes in rats. Horm Metab Res 24: 508-510, 1992.
02 December 2022 3,748 3 View
Please let me know how to obtain macrophage from blood? I need a reliable method.
28 November 2022 7,748 3 View
Are there free databases of peptides?
24 November 2022 3,526 3 View
I'm picking up an old project started before I joined the lab...My lab has cells that were fixed in 10% formalin in March 2020 (almost three years ago). Due to the pandemic, the cells were never...
23 November 2022 321 3 View
Hello! I am trying to start a new experiment using 68Ga and 177Lu labeled peptides on LnCaP and PC-3 cells to find out the binding, internalization and uptake values. First of all, the terms are...
22 November 2022 8,386 0 View
The protein structure is only available in wire kind of form. How can I retrieve the secondary structure back?
22 November 2022 1,150 5 View
Typically, peptides are quantified by MRM by using softwares such as Skyline and Fragment ion library prepared by untargeted approaches are used for that. Is it okay if I quantify peptides...
14 November 2022 6,911 0 View
After extraction of pure essential oil, I dissolved 10% EO in pure DMSO. When I use 10% of that for anti fungal assay in 96 well plate, activity was good but control DMSO also showed activity...
05 November 2022 2,270 14 View
Hi (Salam), I'm studying the expression of some proteins in cell lines using LC-MS. In order to do so, I'll go with the "bottom-up" approach. After selecting the suitable signature peptides, I...
30 October 2022 1,365 2 View