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Questions related to Peptides
Why am I getting a single band at 38 Kda for total p38 but two bands for phospho p38, one at 38 Kda and the other at 28 Kda? Is my sample degrading?
18 September 2025 3,671 0 View
Relay coils are popular means to extend the range of power transfer, can it be called as parasitic elements or not?
31 August 2025 5,988 0 View
Also does anyone use nutrient agar half strength? Do we need to add extra agar for firmness?
14 August 2025 1,581 3 View
I have generated stable knockdown MCF7 cells of a protein using lentivirus and observed slower growth and inhibition of Raf-MAPK pathway. I want to understand if I can do IP from WT MCF7 and KD...
06 August 2025 5,647 0 View
I am currently working on an immunofluorescence experiment in spleen of mice in which I use an antibody conjugated to FITC. However, I am experiencing autofluorescence from red blood cells, which...
20 July 2025 8,713 1 View
Hi, I usually go for PBS with anti-CD3 coating 24h prior to human CD4+ T-cell activation for further functionality test (proliferation, phenotyping, polarisation, etc.) That is what I saw other...
09 July 2025 9,579 2 View
Hi，everyone，as we know, antibodies like anti-CD3 and anti-CD28 are commonly used to activate human T cells in vitro. I’m wondering, can these same antibodies also activate Jurkat cells? Basically,...
03 July 2025 2,903 2 View
Which can reliably determine size/polydispersity of sonicated liposomes (~200 nm target) for an undergraduate biotechnology project? Preferably low-cost and equipment-limited.
02 July 2025 8,342 5 View
Hello Everyone , I am currently working with endothelial cells, marked with GFP, I took picture with microscopy every day and wanted to quantify the organization in the scaffold compared to the...
26 June 2025 3,568 0 View
Reputable publishing firms like Elsevier, Springer, Sage, and Wiley look for several key factors when considering a journal for publication. These factors include international focus, a strong...
20 June 2025 3,632 1 View
My study aims to asses soil microbial diversity across a range of agricultural soil managed under various agricultural practices.
10 June 2025 7,540 2 View
Genetic sequence (nucleotide or protein) placement (GenBank)
10 June 2025 9,560 0 View
Cryptocurrencies offer diversification benefits due to low correlation with traditional assets. However, their high volatility, regulatory uncertainty, and lack of intrinsic value pose risks....
30 May 2025 8,661 2 View
I used R18S3+MTG cryopreservation media to store sperms, obtained from the cauda epididymis of male mice. After one week, I thawed the sperm from liquid nitrogen, using a 37 C water bath. I...
29 May 2025 2,133 1 View
I am going to use PLGA for my dissertation, hope you can help me look for supplier that sell small amounts or any one who wants to donate I am willingly accept it.
28 May 2025 9,964 3 View
Also, discuss how much dilution is needed? How does this dilution affects the QY? The carbon dots will be in a liquid form.
26 May 2025 9,481 1 View
we use Zobell marine agar media
22 May 2025 444 2 View
Regarding the use of Collagen, rat tail (Cat.NO.: HY-NP010, bought from MCE) to promote cell attachment on thin-layer coated culture plates, I would like to ask the following details: 1. What is...
15 May 2025 9,576 1 View
I want to stabilize my gelatin structure by EDC/NHS crosslinking so can I just use it with PBS buffer ?
12 May 2025 5,385 2 View
Hi everyone, I would like some advice on the best method for treating Arabidopsis leaves with elicitors to understand the defense response. Which approach is generally more effective? – using...
10 May 2025 1,269 1 View
why does this happen in my cinnamon oil encapsulated PLGA nanoparticles
02 May 2025 3,596 2 View
Good day, everyone! Our team has problem with selection PCR condition. We do not have electrophoresis equipment and can not detect PCR product using this method, only Real time PCR. Please see the...
24 April 2025 248 12 View
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23 April 2025 2,317 0 View
Will the induced voltage in the receiver coil remain same or will it degrade upon flipping the receiver coil upside down?
22 April 2025 9,793 3 View