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Questions related to Peptides
I'm having trouble obtaining a well-defined band of the COI gene in mollusk (Aulacomya atra) adductor muscle samples, despite using 35 cycles and TBT-par. Other mitochondrial and nuclear genes...
15 August 2023 7,985 2 View
I am trying to extract DNA from a single insect from different sites. After extraction nanodrop readings show around 12-15 nanogram per microlitre concentration which is normal for the insect....
14 August 2023 5,142 4 View
Polymerase Chain Reaction (PCR) testing is a molecular biology technique used to amplify and analyze DNA or RNA samples. So, To perform PCR testing, which list of laboratory apparatus and...
14 August 2023 2,488 4 View
So I have tried to PCR a fragment for my assemblies. (not so successful as of late I might add) although I have done them almost 100 times. I have changed polymerases (not related to my...
12 August 2023 7,875 0 View
I work in a single-molecule analysis lab. I have been waiting on a supplier to send sodium bicarbonate for a while, and I want to begin experiments now. We have a stock of sodium bicarbonate from...
10 August 2023 8,251 5 View
We synthesized a peptide made up of 7 amino acids. After cleaving the peptide with TFA, we tried to precipitate the peptide from cold ether. However, it seems like our peptide is somehow soluble...
09 August 2023 226 3 View
Hi everyone, I am enriching phosphopeptides with TiO2 beads. I only use very manual like using eppendorf to combine with sample and TiO2 , then centrifuge to remove the supernatant and elute...
09 August 2023 4,490 6 View
I would like to know if the binding between extracellular vesicles (EVs) in serum and immunoglobulins is strong or not.
03 August 2023 6,277 1 View
I want custom synthesized mouse SIRPα siRNA. Can anyone please suggest some good papers where I can find mouse SIRPα siRNA sequence?
31 July 2023 3,730 1 View
Why is biodiversity important to climate change and why it is too narrow to define the biodiversity crisis as simply a loss of specie?
30 July 2023 3,105 6 View
I use gel 16.5%, 120V, 10 µL sample. Thank you
26 July 2023 2,168 1 View
I would like to isolate RNA from plants, is an low cost RNA isolation protocol available? Preferable using Chloroform as main reagent. Thank you in advance, Kiriakos Athanasiadis
25 July 2023 8,010 2 View
CID problems and solutions in LC-MS/MS analysis.
22 July 2023 8,183 2 View
I am trying to dimerize a synthetic peptide (22 amino acids) with a N-terminal cysteine, that was added for this purpose. I use the BM-(PEG)3 crosslinker from Thermo Fisher, which is based on...
20 July 2023 9,367 6 View
Immunofluorescence on PFA-fixed tissue
17 July 2023 6,599 4 View
Hello researchers and experts, I hope this post finds you well. I am currently conducting research in the field of drug design and (POM) theory. I am excited to connect with fellow researchers and...
12 July 2023 534 2 View
Hello, I'm Sanam , a grad student new to ITC. I am trying to do titration between a protein and small molecules that we knew it binds to the protein since it's a commercial inhibitor. I purified...
08 July 2023 293 5 View
I have protein extracts from samples of testicular tissue from Wistar Rats, that were lyophilized contained RIPA buffer with protease inhibitors (150 mmol NaCl, 1% Triton X-100, 1% sodium...
08 July 2023 4,428 3 View
Hi, I was wondering if it is possible to set up an HPLC system by adding a fluorescence detector from a different manufacturing company than the HPLC. I have an Agilent HPLC in the lab equipped...
29 June 2023 8,022 1 View
I am determining the in vitro release profile of citral from chitosan nanoparticles. I will be using the dialysis method using PBS at different ph as the release buffer. During the dialysis, once...
27 June 2023 9,230 2 View
Hello all, I am in the process of setting up an industrial enzyme production facility, initially for producing poultry feed enzymes. Does anyone have a contact of where I can procure the strains...
26 June 2023 6,430 1 View
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14 June 2023 4,897 3 View
My experiment includes synthesis of polymeric conjugate using hyaluronic acid and a flavonoid molecule in DMSO. Followed by purification of the conjugate by using dialysis membrane (MWCO- 12KDa)...
12 June 2023 1,159 3 View
in MTT assay we use DMSO as solvent ,, but for how long? and is it necessary to be kept in dark place ?
06 June 2023 7,510 5 View