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Questions related to Peptides
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
04 March 2021 6,331 3 View
The computational cluster I have been submitting jobs is switching clusters from one that supports 24 ncpus to 36 ncpus. The problem is, now when I run my jobs it produces the following error: 36...
01 March 2021 728 3 View
Hi everyone, I am a master student who currently working with a program detecting amyloid β aggregation, so I need to use Abeta 42. After treating with HFIP and get a peptide film, I want to...
24 February 2021 4,255 3 View
Hello, I am learning how to isolate thioglcollate primed peritoneal macrophages from mice but my yield has been lower than expected. I expect a total yield of 1x10^7 but I have had inconsistent...
23 February 2021 2,164 4 View
This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...
22 February 2021 779 3 View
I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...
22 February 2021 4,762 3 View
I'm currently searching for vendors to provide L-cystine dihydrochloride for use in plant and animal cell culture media. I'm attempting to obtain a version with no more than 0.5% loss on drying;...
08 February 2021 4,355 2 View
Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...
04 February 2021 2,184 3 View
I have been synthesizing peptides on resin beads. I am wondering what best storage method is there to store the beads after synthesis before cleaving. And how long can they be stored in that...
03 February 2021 5,763 5 View
I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...
03 February 2021 4,031 1 View
Hello, everyone! I have chemically synthesized antioxidant peptide isolated from the plant. I have to validate its bioactivities in Adult zebrafish. I have to administer this peptide to zebra...
02 February 2021 5,946 3 View
I am synthesizing 7 amino acid long peptide sequence with CTC resin, 50oC coupling for 10 min. On cleavage (TFA/TIPS/Water 95:2.5:2.5) I got peptide with good yield. However, It has 68 Da more...
31 January 2021 8,658 3 View
Basically, I want to get the sequence of peptides. I assume, digesting these peptides from each band may give me a different sequence than the original through LC-MS analysis. If digestion doesn't...
31 January 2021 5,621 3 View
Right now, I am using ProtParam to compute GRAVY index of peptides. However, some of my peptides are post-translationally modified, and contains unusual amino acids. I assume that ProtParam does...
30 January 2021 8,513 4 View
Hi everyone! I am purifying a peptide from a mixture of proteins. The peptide (3 kDA) is in a solution containing a high concentration of urea and imidazole. I would like to be able to change the...
28 January 2021 4,145 4 View
Hi all Scientists; I have similar question. Can you please help me in selecting unique/distinct peptides in case of LC-MS/MS analysis? I have done salivary gland proteomes for...
27 January 2021 7,634 3 View
Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...
26 January 2021 8,546 3 View
Greetings In a theoretical perspective, I want to use a human-derived peptide as a plausible antigen but the problem associated with tolerance hampers my approach. Then I thought why not denature...
21 January 2021 2,380 1 View
Hello everyone, I would like to get help about finding any server or application which can give me info about free energy and dissociation constant of protein-protein docking outputs. I have...
16 January 2021 681 3 View
We are using Proximity ligation assay extensively, primarily by using Duolink secondary antibodies from Sigma\Merck. In the past few weeks they ceased to produce secondary antibodies with primers...
14 January 2021 5,631 1 View
Recently, I try to obtain disaccharide peptides and lactoyl peptides from Mycobacterium Smegmatis, but I cannot succeed. My method is: Disaccharide peptides: The mycobacterial strains were...
13 January 2021 8,489 3 View
I have been using PyRx to dock peptides to a protein. Initially the numbers were small so it was okay. But now I have to dock over 500 peptides and doing so manually would take forever. My...
11 January 2021 3,466 4 View
I have identified peptide sequences of SARS-CoV-2 by in-silico method. I am attempting to validate all these sequences with wet lab mass spectrometry based peptide sequences. SARS-CoV-2 specific...
23 December 2020 3,523 3 View
SPPS of a 16 aa peptide was performed on a 0.15 mmol scale. Resin gained a minimal amount of weight: starting weight of 224 mg and a final weight of 280.I believe my peptide failed to elongate but...
22 December 2020 9,551 11 View