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Questions related to Peptides
What is the role of water management in climate smart agriculture practice and regenerative development as a natural solution for sustainability?
15 October 2023 6,437 2 View
Have been finding out possible HIV exposure in food within California is successful on signs/ symptoms appearing; WHAT IS CONCENTRATION AMOUNT FOR THIS TO BE POSSIBLE?
14 October 2023 3,210 2 View
I am planning to perform C13 MFA, and I was wondering how I can weigh the labeled glucose and add it to the media while maintaining sterility. I didn't find any protocol specifically explain this...
13 October 2023 1,920 2 View
Is it possible to hypothesize the existence of a universe governed by supersymmetric natural laws that existed before the Big Bang (Garden of Eden)? Is it possible to believe that the Big Bang...
06 October 2023 292 6 View
I'm working on SH-SY5Y viability test with MTT assay, using vitamin C and H2O2. In our lab protocol, after 30-48h from cell seeding it reaches 70% confluency and gets ready for cell deprivation....
04 October 2023 9,539 3 View
Dear all, I am wondering if you have ever used (HLA-transfected) HEK293T cells in co-culture with HLA mismatched PBMC to study alloimmune responses? Do you think co-stimulation in addition to...
27 September 2023 366 2 View
I am attempting to amplify nirS and nirK in some salt marsh sediment samples for my Masters thesis studying denitrification. My nirS gene is amplifying great in both PCR and qPCR. In the...
27 September 2023 6,889 3 View
Good day to whom it may concern! I wanted to build a simple PEM electrolyzer, could you suggest a good synthesis for the proton exchange membrane? I work in a electrochemical laboratory, so...
26 September 2023 4,458 1 View
I want the home-made recipe of denaturation solution (5x per ug protein), for the "Endoglycosidase H - Sigma-Aldrich". Thank you in advance.
14 September 2023 787 0 View
in other words is it possible that there are bacteria containing two different types of plasmids in a single bacterial colony?
13 September 2023 3,054 2 View
There are someone here who use fluoracle software for fluorescence measurements? I want to know if you do baseline before measurents or how do you remove the fluorescence peacks of solvents
12 September 2023 7,209 0 View
What are the things that need to consider when determining antimicrobial susceptibility testing?
03 September 2023 9,268 2 View
The question is related to a medication list, which I have embedded into a table. Current medications are asked at multiple follow-up points; however, I want participants to have the table...
23 August 2023 8,667 1 View
I'm researching a way to calculate intraspecific size variation for sauropods in general, and Camarasaurus appears to be the best sample population. Ikejiri 2004 has provided some useful...
23 August 2023 6,496 2 View
What are the climate change adaptation strategies in agriculture and strategies for developing climate resilient crop varieties?
23 August 2023 6,246 2 View
Meta-heuristics are methods used in solving optimization problems (OPs) in several areas. They follow some rules and instructions, and there is no learning from data in order to solve the OPs....
22 August 2023 10,012 0 View
Methanol and ethanol plant extracts of lemongrass, centella asiatica and moringa oleifera not showing activity in the varied concentration of lower to higher concentration 300mg/ml, 500mg/ml and...
16 August 2023 5,432 3 View
I'm having trouble obtaining a well-defined band of the COI gene in mollusk (Aulacomya atra) adductor muscle samples, despite using 35 cycles and TBT-par. Other mitochondrial and nuclear genes...
15 August 2023 8,060 2 View
What mediating factors affect the relationship between 21st century skills and expectancy-value?
15 August 2023 8,613 1 View
I am trying to extract DNA from a single insect from different sites. After extraction nanodrop readings show around 12-15 nanogram per microlitre concentration which is normal for the insect....
14 August 2023 5,279 4 View
Polymerase Chain Reaction (PCR) testing is a molecular biology technique used to amplify and analyze DNA or RNA samples. So, To perform PCR testing, which list of laboratory apparatus and...
14 August 2023 2,596 4 View
I used the ExoSpin kit to isolate EVs from Plasma using 0.22 double filtration and 10KDa Ultrafiltration device and resuspended in PBS (cacl and mg free) and still find that particles are clumping...
14 August 2023 4,847 0 View
So I have tried to PCR a fragment for my assemblies. (not so successful as of late I might add) although I have done them almost 100 times. I have changed polymerases (not related to my...
12 August 2023 8,001 0 View
I work in a single-molecule analysis lab. I have been waiting on a supplier to send sodium bicarbonate for a while, and I want to begin experiments now. We have a stock of sodium bicarbonate from...
10 August 2023 8,362 5 View