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Questions related to Peptides
We are planning to use 4-SULFAMYLBUTYRYL NOVASYN TG RESIN (catalog no - 8550440001). Please suggest us if we can use microwave coupling method against this resin or not? Will there be any problem...
27 May 2023 8,655 0 View
Hello all, first post on ResearchGate! I have been performing immunoprecipitations with FLAG beads (abcam, ab270704), eluting using SDS loading buffer (with DTT) and boiling. The eluted products...
23 May 2023 9,016 5 View
Hi everyone, I'm carrying out a peptide coupling reaction, with the coupling reagents being N,N'-Diisopropylcarbodiimide (DIC) & hydroxybenzotriazole (HOBt). I'm making glycine to couple with...
17 May 2023 4,990 1 View
We are in discussions with Agilent to acquire a custom mutation and fusion panel. They introduced us to the pre-capture polling solution. I would like to know about the experience of those who...
15 May 2023 9,434 2 View
I am trying to construct a phylogenetic tree between some protein sequence alignments. I am struggling however with the interpretation of which tree I should use as the program seems to make many...
10 May 2023 5,232 1 View
Hello, I am designing a plasmid with an SV40 promoter-driven antibiotic resistance. Does expression from an SV40 promoter require a TATA box upstream of the transcription start site? The...
09 May 2023 1,703 4 View
I´m performing a meta-analysis and some studies report continuous data as percentage of change (pre vs. post) but does not report standard deviation from this percentage of change. I´ve been...
05 May 2023 2,277 3 View
Hey all! I have a question about my proteomics data evaluated in Proteome Discoverer. I got three volcano plots of three biological groups in a ratio with control group. In one group I see a...
05 May 2023 6,745 3 View
Open Journal of Clinical and Medical Case Reports (ISSN 2379-1039) (jclinmedcasereports.com) Not indexed on PubMed but NLM/PubMed ID: 101658769 (citations only) It claims Impact Factor:...
04 May 2023 925 1 View
I tried to detect the endogenous expression of my protein of interest, but I can't. However, at the same time, I can verify the overexpression of the protein with different antibodies. I tried...
03 May 2023 3,519 4 View
We are using Mobitech bacillus system but that is not working
28 April 2023 2,627 0 View
I performed crosslinking using a 10 mg pellet and 5 ml of diluted 2.5% glutaraldehyde for a period of 2 hours. Upon attempting to dissolve the crosslinked enzyme for activity assay, I encountered...
28 April 2023 6,020 3 View
Hey everybody, I am looking for an easy method and also software for analyzing TCR repertoires. is Spectratyping the easiest method? do you suggest any method which is easy to set it up in the...
25 April 2023 9,803 4 View
Anatomy and movement assignment 1 sub question
24 April 2023 6,965 3 View
Can a drug show cytotoxicity at 24 hours time point but cannot show cytotoxicity at 48 hours in MTT assay? Or there is a possibility that somewhere the experiment has gone wrong? The mode of...
20 April 2023 4,428 1 View
How do I measure activity of an enzyme if it catalyses a two-step reaction? The final product is what I want.
19 April 2023 287 7 View
I do not sure whether FⅨa is one of the Serine Protease Family. I found the date said FⅩa and FXIa are belong to Serine Protease. My question is expect the spectrozyme fⅨa that cansed as a...
19 April 2023 1,568 1 View
The lower concentration that i did was 1 ppm and the latter was 10 ppm.
17 April 2023 5,422 1 View
For protein extraction i found a protocol that separate phosvitin from egg yolk using ultrafiltration trough 10 MWCO then uses the filtrate. But phosvitin molecular weight is 35 KDa. So how it is...
17 April 2023 4,274 2 View
I want to design a drug delivery system using peptides as targeting ligands which are supposed to interact with cell surface receptors. Has anyone experienced a similar experiment to help with the...
16 April 2023 6,848 0 View
Hi, I did western and you can see my beta actin bands. I put 10 uL primary antibody for my interesting protein, but my professors said 25 uL should be. I donot understand. Wouldn't I have seen a...
12 April 2023 8,603 2 View
I am trying to covalently attach the RGD peptide sequence to PNIPAM-co-AAc microgels already coated on different substrates (Si, Glass and Polyurethane). I am going to use the common carboxylic...
11 April 2023 7,991 0 View
BiFC（bimolecular fluorescence complementation） is a useful method to study protein interaction. But what makes me surprised is that the usage is enriched in plant research field. I have no...
04 April 2023 2,579 2 View
Specifically, I am wondering if I can predict the surface marker expression of CD14 and CD16 on monocytes based on scRNAseq data that includes the expression level of those two genes. Does anyone...
30 March 2023 6,785 3 View