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I am doing it By hydrothermal method.
14 April 2025 9,047 9 View
I'm relatively new to the chronic illness space, so I'm clearly looking in the wrong places. I cannot find clear scoring instructions for the Revised Illness Perception Questionnaire (IPQ-R)...
14 April 2025 9,599 2 View
I have purchased DNAse 1 enzyme from MP biomedicals (Cat no 100575). It is a 20mg package and ≥2000 kunitz U/mg is mentioned on the vial. In which buffer/solution should I be dissolving it? I...
03 April 2025 9,086 1 View
I want to do ChIP-Seq on human muscle samples (stored at -80°C). Can anyone provide me with a written protocol to start ChIP from muscle tissue, or a paper to start from? Additionally, since we...
18 March 2025 4,603 0 View
I have ionic liquid and H2, CO2 gas, catalysts. I want to do the hydrogenation reaction in a high pressure reactor but I dont know how to feed the reactants then start the experiments. Thank you...
13 March 2025 5,357 4 View
Hi, I have extracted DNA from some cells and eluted in buffer AE (Qiagen) which came with the kit. After this I have diluted the sample in nuclease free water to make up a specific concentration...
13 March 2025 9,193 5 View
AI and ML are widely used in HFT to improve trading efficiency and market prediction capabilities. However, AI trading systems still face challenges with market noise, black box problems,...
13 March 2025 5,830 2 View
JAS ChatGPT LLM (BASIS_Definition: Copy & Paste) Mapping the Path to Graceful Human Evolution I. Introduction This document provides a comprehensive framework for integrating social,...
14 February 2025 5,426 4 View
What would you do if a journal keeps your manuscript for six months, ignores your repeated enquiries about the status and even ignores a request to withdraw the paper? Is it considered as double...
10 February 2025 3,368 4 View
Hi all. I am trying to fuse 2 fragments into 1 using overlap extension PCR. The lenght of the fragment 1 around is 3kb and fragment 2 around 300bp. All 2 fragments are derived from previous PCR...
29 January 2025 3,264 1 View
For molecular docking databases like Pubchem can be used for downloading ligands in 3D form but unfortunately most do not give a very good docking score. However when using tools like Chemdraw to...
29 January 2025 3,176 0 View
Hello, I am trying to culture a specific subset of macrophages from murine pancreas, but they do not grow well in cell culture with RPMI-1640 (+FBS + M-CSF). I want to try growing them with some...
14 January 2025 2,972 2 View
I need to detect Human soluble a-Klotho in mice tissue homogenates. The kit (Human soluble a-Klotho ELISA Kit (IBL, Cat# 27998)) works well with plasma and serum, manufacturer also mentioned urine...
07 January 2025 6,151 0 View
I am new to CRISPR and we are trying multiplex genome editing in plants for 3 guideRNAs in a vector for a gene. We are followingA CRISPR/Cas9 toolkit for multiplex genome editing in plants...
16 December 2024 2,479 2 View
Intercropping the two crops good in terms of virus disease susceptibility
15 December 2024 6,752 2 View
Hi! I’m currently evaluating the best conditions for handling and storing a bottle of (3-Aminopropyl)trimethoxysilane (APTMS). Before opening it, I’d like to seek practical advice based on your...
01 December 2024 3,678 0 View
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30 November 2024 8,268 0 View
Hi everyone! I’m analyzing the microstructure of the coarse-grained heat-affected zone (CGHAZ) of welded EH36 steel, and I’d love to hear your expert opinions. Based on the literature and my...
28 November 2024 5,100 0 View
Greetings from JSS Medical Research! We are reaching out to request access to the validated REQUEST™ questionnaire as we understand you were one of the authors of this validated questionnaire...
26 November 2024 3,473 0 View
Hello, everyone. I have a strange problem during MCAO surgery on a transgenic mouse colony. I performed MCAO on the left side, which should have resulted in damaging the right side, yet the same...
26 November 2024 2,905 0 View
Hello! I'm new to using AFM to analyze nanoparticles in a solution, and I have a question. I see a "line fit 2.79 nm" bar on the right side of my image. Does this refer to the height of the...
21 November 2024 7,669 5 View
A nano formulation containing excipients like TEA, Carbopol, lactic acid etc. is found to be stable in Realtime and accelerated stability studies. It it necessary to check compatibility of the API...
19 November 2024 758 5 View
Dear experts and researchers, Currently, I have found that the Greenwood contact models and the Persson contact theory are mainly two parallel valuable theories. I wonder if it is feasible to...
18 November 2024 8,821 2 View
I have added images from internet to my review paper. How to get copyrights permission to it?
03 November 2024 10,029 1 View