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Questions related to Peptides
We synthesized a peptide made up of 7 amino acids. After cleaving the peptide with TFA, we tried to precipitate the peptide from cold ether. However, it seems like our peptide is somehow soluble...
09 August 2023 277 3 View
Hi everyone, I am enriching phosphopeptides with TiO2 beads. I only use very manual like using eppendorf to combine with sample and TiO2 , then centrifuge to remove the supernatant and elute...
09 August 2023 4,551 6 View
I would like to know if the binding between extracellular vesicles (EVs) in serum and immunoglobulins is strong or not.
03 August 2023 6,327 1 View
Hi, I would really appreciate if somebody with experience on working with Melatonin could help me. I would like to analyse melatonin in plasma. I need to detect low concentrations. I have read...
03 August 2023 1,076 0 View
Does herbicidal residues used during termination of cover crops have impact on soil health? Other alternatives for cover crops termination may have benefits over use of herbicidal residues.
02 August 2023 9,846 1 View
I want custom synthesized mouse SIRPα siRNA. Can anyone please suggest some good papers where I can find mouse SIRPα siRNA sequence?
31 July 2023 3,767 1 View
Why is biodiversity important to climate change and why it is too narrow to define the biodiversity crisis as simply a loss of specie?
30 July 2023 3,172 6 View
I would like to get rid of surface proteins of fixed cells, but I am concerned about the detachment. Does anyone have any experience? I was thinking about low trypsin C, short exposure and using...
28 July 2023 4,061 0 View
I use gel 16.5%, 120V, 10 µL sample. Thank you
26 July 2023 2,216 1 View
I need a database of FDA approved drugs or compounds for my research in medicinal chem
26 July 2023 9,289 3 View
I would like to isolate RNA from plants, is an low cost RNA isolation protocol available? Preferable using Chloroform as main reagent. Thank you in advance, Kiriakos Athanasiadis
25 July 2023 8,074 2 View
CID problems and solutions in LC-MS/MS analysis.
22 July 2023 8,225 2 View
I am trying to dimerize a synthetic peptide (22 amino acids) with a N-terminal cysteine, that was added for this purpose. I use the BM-(PEG)3 crosslinker from Thermo Fisher, which is based on...
20 July 2023 9,429 6 View
Immunofluorescence on PFA-fixed tissue
17 July 2023 6,644 4 View
How the difference in plaque morphology of a bacteriophage affects the adsorption time and latency period to its indicator host?
15 July 2023 3,561 1 View
MAP Tau, htau40, 2N4R, has the actual weight of 45 kDa but runs as 67 kDa on SDS-PAGE. What can explain this much weight difference? Is it specifically about Tau's unique structure effecting...
14 July 2023 3,789 1 View
Hello researchers and experts, I hope this post finds you well. I am currently conducting research in the field of drug design and (POM) theory. I am excited to connect with fellow researchers and...
12 July 2023 580 2 View
Hello, I'm Sanam , a grad student new to ITC. I am trying to do titration between a protein and small molecules that we knew it binds to the protein since it's a commercial inhibitor. I purified...
08 July 2023 347 5 View
I have protein extracts from samples of testicular tissue from Wistar Rats, that were lyophilized contained RIPA buffer with protease inhibitors (150 mmol NaCl, 1% Triton X-100, 1% sodium...
08 July 2023 4,469 3 View
I want to find out the mutation sites from the whole genome sequence of one of the bacterial strain so which software will best to use
06 July 2023 5,840 0 View
We identified many cyclotides with an intact mass range of 2-4kDa in cyclotide-rich extract. Can we able to encapsulate cyclotide-rich extract with 2-4kDa mass range in a nanoparticle drug...
05 July 2023 3,820 0 View
Did someone used specific blocking antibodies that target the adhesion molecules involved in the interaction between hematopoietic cells and MSCs (specifically antibodies against VCAM-1 or α4β1...
04 July 2023 2,524 0 View
Hi, I was wondering if it is possible to set up an HPLC system by adding a fluorescence detector from a different manufacturing company than the HPLC. I have an Agilent HPLC in the lab equipped...
29 June 2023 8,065 1 View
Hello, I have a data set with N = 369 individuals measured at a single time point. The goal of the study is to create an assessment of psychological safety (PS). The assessment is a self-report...
29 June 2023 1,962 3 View