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Questions related to Peptides
I would like to present isolated yeast genome comparative with S. cerevisae S288c and present on circular visulization. So what's program and how did it. ? Ps. I'm biologist and haven't basic...
27 October 2022 7,012 4 View
A molecule inhibits both single and double-standard binding proteins. What could be the most probable mode of action of this molecule? Protein or DNA? What are the possible methods/ experiments to...
19 October 2022 2,613 0 View
Dear all, I install Maxquant a few days ago,but when I run my project, it keeps popping up this error, so I can't get the result。 I have installed .Net 6.0, but it is not work.Could anyone please...
18 October 2022 2,342 4 View
Ive made a mistake of preparing 0.1M NiSO4 in DURAN glass bottle. Can I reuse the bottle? If so, how can I neutralize the bottle from any traces of NiSO4?
17 October 2022 1,857 2 View
Hi, Is it efficient to use pI precipitation for peptides (actually small proteins; e.g3-4 kDa)? Could you provide references or share your personal experiences?... Although it is a versatile...
14 October 2022 4,824 1 View
Experimental set-up: I have recorded plant performance values in triplicate on two plants from a 4x3x2 factorial designed experiment, over 7 days As I have only two biological replicates per...
13 October 2022 5,491 3 View
I have the results of an organoleptic test using nonparametric data analysis with the Kruskal Wallis Test. Can I continue to analyze the data with the Tukey test?
13 October 2022 533 6 View
When compared the salary with the amount of work post-doctoral fellows usually do in the so-called developed countries it seems seriously mismatched. Many of them end up living hand to mouth....
10 October 2022 2,928 4 View
I wanted to know how to label 1 to 1210 amino acids in the plot and how tick out 231 positions from 1210 amino aids. Thank you!
15 September 2022 3,533 0 View
dear colleagues , this question could be not simple , I want to ask that which are the mechanisms could explain for the swollen macrophage after being infected ? the negative control showed...
07 September 2022 926 0 View
I'd like to know how to track/follow the flow path through the sample pump, to the column and into our fraction collector in case of AKTA Avant? I mean what are the different methods/Techniques...
07 September 2022 8,890 0 View
I synthesized two chemical probes with masses (C24H36N6O2 : 440) and (C24H32N6O2 : 444) as light and heavy probes, respectively . The idea is to use these two probes to label and quantify protein...
01 September 2022 8,071 0 View
I had a ready calculation table for my sequence that I made a peptide, but I wanted to understand the formula behind this calculation if I have 5 equivalent of Serine with its molar mass 383.4...
21 July 2022 2,729 4 View
Do someone know some strong counterions that are not perfluorated for reversed phase chromatography coupled to mass spectrometry ? We already used perfluoroheptanoic acid in a context of...
24 June 2022 4,007 1 View
In wet lab experiment I got the sequences of Heavy chain and the Light chain. I used AlphaFold-2 to generate their the structure PDBs. Now the challenge is how to merge them to get one PDB? Not...
17 June 2022 4,075 5 View
Hi everyone and expert glycobiologists! I'm trying to optimize an assay that uses PNGaseF (N-glycosidase; New England Biolabs P0705S) to deglycosylate my cells (Jurkat T-cells). However, I cannot...
05 May 2022 1,486 0 View
Hello, I was doing TEV cleavage and I accidentally added a 1:1 ratio of TEV protease to my protein(His_GGSGGS_TEV cleavage site_target protein)...Our lab use to add a 1:40 ratio of TEV protease...
22 April 2022 5,895 1 View
According to LysM Receptor-Like Kinase and LysM Receptor-Like Protein Families: An Update on Phylogeny and Functional Characterization by Buendia et al (...
10 March 2022 7,296 0 View
Hello, I want to use Rose Bengal for photodynamic therapy, but I need coupling to a protein. I know that rose bengal-hexanoic acid must be synthesized first and then conjugated with the NHS, but I...
01 February 2022 5,699 0 View
Are structure-based peptide drug design and ligand-based peptide drug design mutually exclusive? If we have the crystal structure of the protease and design the peptide inhibitor based on the...
25 January 2022 4,553 7 View
Greetings all. As you probably know the "Tryptone" used as component of LB media and alike are the product of digestion of casein. Similarly Cas-Amino acids are the product of acidic hydrolysis of...
10 January 2022 1,939 1 View
I have already tried different conditions (temperature, salt concentration, presence of reducing agents, different concentration of protease) but the removal of the tag is always very partial (...
06 December 2021 2,119 13 View
Hello, I would like to analyse a peptide by LC-MS/MS. I am not experience in peptides. I would really appreciate if somebody could help me. 1. I have the sequence of the peptide and I would like...
14 November 2021 7,882 2 View
Hello, How can I find amino acids within 6 angstroms of a ligand in Autodock or Discovery Studio? 🌾
06 October 2021 1,094 11 View