Are there mathematical or statistical processing of satellite imagery or digital elevation data in the detection of historical buried rivers?

In the lands with ancient plain sediments, the courses of rivers change dramatically over time for easy movement and the arrival of rivers to an advanced geomorphic stage. Are there mathematical...

02 January 2021 3,098 3 View

Detection of old river streams?

What are the methods of detecting the ancient streams of rivers using remote sensing, and do the satellite images achieve the possibility of detecting and tracking the change.

02 January 2021 5,213 4 View

As a researcher, do you consider yourself a designer?

Researchers go through lengths to convey technical information to both fellow academics and the public. Many struggle with the tools and technologies to effectively make this happen, and others...

20 April 2020 9,049 17 View

Can author published his or her old prospective study in a peer review journal?

Can author published his or her old prospective study in a peer review journal? For example 20 years ago.

13 February 2020 9,717 5 View

What ratio of TEMED is necessary in N-isopropylacrylamide polymerization?

I am working on NiPam polymerization and I am unsure of the necessary ratio of TEMED necessary to achieve the correct polymerization ratio for the reaction to occur. I am unable to use nitrogen...

12 November 2019 4,499 3 View

What is the more interest topics in smart city today's?

I wish to write an article in a smart city.

09 October 2018 6,197 1 View

A man builds a house with all 4 sides facing south. A bear walks past the house. What color is the bear?

A man builds a house with all 4 sides facing south. A bear walks past the house. What color is the bear?

02 March 2018 6,606 2 View

A bear falls into a 5 m deep hole and hits the bottom of the hole after 1.00861 seconds. What is the color of the bear?

A bear falls into a 5 m deep hole and hits the bottom of the hole after 1.00861 seconds. What is the color of the bear?

02 March 2018 3,865 0 View

Could you please tell me how to measure alpha-amylase activity by Dinitrosalicylic Acid Method (DNS)?

Biochemists, Microbiologists, Dear colleagues, I want to measure alpha-amylase activity by the Dinitrosalicylic Acid Method (DNS) but I have a problem. Could you please tell me how to do, how and...

01 October 2017 5,533 4 View

Why I can not obtain a clear solution of ZnSO4 with water?

08 September 2017 4,093 5 View

Hello, how can i find a primer to identify Bacillus Sp. with PCR? Can someone help me with this?

I am looking for PCR primers for bacillus Sp.

15 February 2021 2,552 5 View

the product size is 191bp would it be a problem during conventional pcr?

I have designed a primer for Bacillus subtilis sfp gene. As I have mentioned the size is 191 bp. however I need some suggestions regarding PCR temperatures and cycle length. As the it is smaller...

09 January 2021 8,309 4 View

No title

Hi, I am interested to see the effect of BCG on macrophages (microglia )Therefore , I would like to know the amounts and the routes of administration which is safe in mice and how long is...

20 December 2020 7,166 2 View

How to handle milk clotting Issues during media preparation for Screening of Halotolerant Protease Producing Bacteria (with Skim Milk)?

We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...

04 December 2020 7,657 6 View

Best way for knockout of gene in Bacillus genome?

Hi everyone! I would like to knockout a gene in the genome of Bacillus. To do that I tried to make a construct that has 1) a backbone, 2) a kanamycin resistance cassette, flanked by 3) the...

24 November 2020 489 1 View

How can i get nonsporulating bacterial bacillus subtillis strain spoIIE without antibiotic growth conditions ?

10 November 2020 7,168 4 View

Can someone help me to understand IGEM 2016 protocol to Bacillus subtilis transformation?

I'm trying to transform Bacillus subtilis to fluorescence, and I will use this IGEM protocol with medium MNGE, but there's a part that I can't understand. It says: "Measure the OD600 of your...

29 October 2020 2,043 1 View

How does the spores of Bacillus anthracis cause the anthrax disease and how can it be weaponized?

Anthrax is a disease caused by aerobic spore-forming Bacillus anthracis. This spore can resist environmental changes and resist biological extremes of heat, pH, chemicals, and other factors; it...

20 October 2020 4,658 8 View

Can I transform Bacillus Subtilus with ligation reaction directly?

Hi All, I want to transform bacillus subtilus with ligation reaction directly. Is that possible? To be more clear, I have a shuttling plasmid between bacillus and e.coli. I know usually we need...

14 October 2020 1,743 1 View

I am not able to find the best working protocol for making electrocompetent cells for bacillus sp. Can anyone help me with this?

I am using a protocol for Bacillus subtilis for which there is a requirement for special electroporation media having sorbitol, mannitol and glycerol. I am using puc18 plasmid (conc...

16 July 2020 3,417 2 View