I'm trying to solve an assignment question where we are given a Amino Acid sequence and was asked to use this a basis to design probes and select clones?
The aminoacid sequence is S-N-W-D-Q-A-M-G-L-F-V-I-R-P-C-E-H-Y-K-T I used a online tool EMBOSS Backtranseq to backtranslate from protein to nucleic acid.
This is the sequnce which I got AGCAACTGGGACCAGGCCATGGGCCTGTTCGTGATCAGGCCCTGCGAGCACTACAAGACC
Can someone explain me why this is the mostly likely sequence of the all possible codons?
Then, when I start to design probes, I'm facing trouble with all the onlines which ask for minimum of about 100 bases to design the probe. Can someone help me please?