Does anyone know the sensitivity of each label digoxigenin (dig), carboxyfluorescein (FAM), FITC and biotin in nucleic acid based lateral flow assay?

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Michael G. Weller

Streptavidin/Biotin is by far the strongest interaction. However, you have to take care that the samples or reagents do not contain any biotin.

Yuanyuan Wu

Since the other labels, DIG, FITC, are antibody-antigen interactions, Michael is right: Biotin-avidin will be the strongest and most sensitive interaction.