The aim is to preserve the most of RNA structures/base pairing after extraction of RNA from virus.
so the question can really be read as: regarding the common RNA extraction methods, what are denaturing, what are not?
I would assume that phenol/trizol, SDS/BME-based virus disruption method are all denaturing?
Would LiCl or EtOH precipitation denature RNA/break RNA structure?
Would spin column denatures RNA to some degree? Maybe the silicon interaction with nucleic acid changes the charge and/or folding of RNA molecules?
Maybe I can proteinase K to strip the capsid, but how do I purify/collect RNA without denaturing?
Thanks
Hi Sourav,
Thank you for the insight. One thing I don't understand: if ethanol can denature the protein by breaking the H bond, why it won't denature RNA by disrupting H bond (base pairing) as well?
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