Why FTIR can not detect bases of nucleic acid in extracellular polymeric substances (EPS)?

06 February 2021 1 5K Report

I characterized functional groups of EPS using FTIR. Goups of sugars and proteins (e.g., -OH, amide, -COOH) as well as phosphate group of nucleic acid were readily detected. However, peaks of nucleic acid bases were not detected, why?

Jun Lu

nucleic acid bases are a series of functional structures. however, FTIR only is utilized to detect the special group. if you want to demonstrate the existence of nucleic acid base, you may consider the amide and alkene as the characteristic peaks

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