I am working on bacterial killing by the antibacterial agent and want to know the mechanism of cell death in bacteria. bacteria show death by cell membrane damage and what will be the probable reason for cell death? Under what conditions bacterial cells will go for apoptosis or necrosis. do both conditions shows protean and nucleic acid leak or only one of them?
Bacterial cells don't undergo apoptosis in the proper sense of the word, since apoptosis is a process that occurs in eukaryotic cells , but not in prokaryotes. Bacteria may be killed due to a range of different causes: blocking of protein synthesis,, damage to the cell wall or the cell membrane etc.
In general, if you know the chemistry of an antibacterial agent..you can predict and explore yourself a mechanism based on literature.For knowing mechanism of cell death in bacteria... We can explore different possibilities like.. Whether a antibacterial is generating ROS... Or lysing cell membrane, destroying biofilms, or inducing ion leakage..
For all the mechanisms, there are corresponding in vitro assays...
ROS-NBT assay
Cell membrane - electron microscopy or live dead assays
Biofilms-crystal violet assay or electron microscopy
Ion leakage-ICP-OES
Apoptosis is a programmed cell death and it has a usuful function.. It could be physiological or pathological..
Apoptosis does not occur in bacteria.
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