How to run a mediation analysis with two different samples?

Hi, I tried to run a mediation analysis in which the DV is made up of test scores after a training in the experimental group VS the control group, and in which the mediator is measured by a...

17 November 2020 7,178 3 View

Using multiple linear regression to solve a multiple-way ANOVA problem?

Specifically, I want to know if I can use multiple linear regression to predict a response value 'y' from it's associated "treatment" or "group" averages in several variables. Let's say:...

04 November 2020 632 19 View

How can I add a chain identifier to a PDB file generated by Phyre2?

I am trying to use DynaMut2 to study stabilizing/destabilizing effects of mutations in a protein. I generated a 3D structure using Phyre2, and it gave me a PDB file without a chain identifier....

22 September 2020 495 11 View

Survey data collection sample purchase - Qualtrics or Dynata?

I am planning for a survey research project (national representative sample for 15 min survey) and am considering which panel/sampling company to use. I have used Dynata before, they are...

22 September 2020 9,334 4 View

Protocol for using Attune NxT Acoustic Focusing Cytometer to detect Ploidy level in leaf tissue?

Hello, I am looking for a detailed protocol to use the Attune NxT Acoustic Flow cytometer to determine ploidy level in plant samples with PI staining. Details for laser, threshold, and...

21 September 2020 8,334 2 View

Can a study have multiple research philosophies ?

I would like to know whether a study can have multiple research philosophy. I am currently studying the influence of ownership on firm performance through regression analysis. I am using case...

19 April 2020 1,073 3 View

How do you define the ontology and epistemology of mixed methods research?

The research which i am doing consists of multiple regression and case studies research. I would like to know whether what i am doing is a mixed methods. Also, what are its ontological and...

08 April 2020 5,457 15 View

Is there any relevant literature detailing extraction methods of simple sugars from plant material for LC-MS analysis?

For my research, I would like to extract glucose, sucrose, and fructose from the crowns of strawberry plants and quantify these sugars using LC-MS. However, I have not had much luck finding a...

11 February 2020 398 3 View

Do viruses mutate more when transmitted to another host than when staying within the first host?

I would like to know if there is a published work studying the within-host mutation rate of a virus versus the between-hosts mutation rate, when a direct transmission event occurred between those...

13 November 2019 5,488 5 View

No title

I have generated a stably transfected SK-N-SH cell line that expresses Amyolid beta 42 fused to the pre-proncephalin signal peptide under de CMV promoter. I checked expression though qPCR and...

23 July 2019 2,685 2 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View