Low yield in total nucleic acid isolation from plasma, why?

03 March 2020 1 3K Report

Hi All,

I've been trying to use the MagMAX Cell-Free Total Nucleic Acid Kit from Thermo to isolate total nucleic acid from plasma, and the plasma was collected from tumour patients. However, I've been getting low yields consistently (less than 10 ng dsDNA with Quibit quantification). Does anyone have similar experience? I'd really appreciate any suggestions to improve the performance.

Many thanks

Lin

Riddhi Thaker

The issue here is that the circulating cell free is already in low fractions.

Maybe try to isolate the nucleic acids when the blood is fresh,

Or see if you can concentrate nucleic acids in the plasma by precipitation step and then proceed with that concentrated plasma.

All the best

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