Dear Sanat Bhadsavle , Dynamic Light Scattering (DLS) and FTIR (Fourier Transform Infra-Red spectroscopy) will give you information from completely different parameters, but probably both of them could provide you useful information about the oligo probe hybridizes well with the target (nucleic acid).

DLS will be useful if this hybrid´s size is substancially different from that of the oligo-probe. It will depend on the original size of the oligo-probe and if it can accomodate just one or lots of aminoacid (I´m assuming the aminoacid is a small molecule). If it just links one aminoacid, it could be that the size variation may be small enough to cause small or not changes at alla on the DLS results, and therefore it would leave you with no clear results.

FTIR should show some change on the IR spectrum of the oligo probe or the aminoacid. If hybridation happens by chemical bonding between these two components you could register the vibrational modes of that bond.

Multiple analytical techniques could be used to know if your hybrid is formed or not, like NMR, Raman or Mass Spectroscopy just to name a few. NMR should show formation of bonds, presence of functional groups... etc. Raman like FTIR should show also vibrational information associated to new bonds or groups. MS should show a larger molecular ion (caused by the add of the aminoacid residue/s to the oligo probe).

Addition of chromophores or fluorophores could also show readily the formation of this hybrids, but require some chemical modification if your components are not naturally colored or show flourescence.

Hope it helps.

Optical properties (surface plasmon resonance) of gold nanoparticles can be monitored before and after the interaction.

Electrophoresis/ circular dichroism spectroscopy can be also performed to confirm the binding.