How can we know the Nucleic acid (DNA) denaturation in sample ?

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Jean-Pierre Jost

The phenomenon of UV absorbance increasing as DNA is denatured is known as the hyperchromic shift. The purine and pyrimidine bases in DNA strongly absorb ultraviolet light. Double-stranded DNA absorbs less strongly than denatured DNA due to the stacking interactions between the bases.

Amarjit K Nath

Measure UV absorbable at 260 nm increase in absorbance indicates denaturation of DNA. Native DNA absorbs less at 260 no.