Suitable PhiX concentration for diversified libraries (MiSeq v2, Nextera XT)?

08 September 2019 1 3K Report

Dear All,

could some please suggest proper PhiX concentration for multiplex Illumina sequencing with use of MiSeq kit v2 and Nextera XT.

samples #1-11 are highly similar - around 50-56%GC

sample #12 significantly different from the others - somewhere around 33%GC.

5% PhiX will be ok?

thanks in advance,

Piotr

Maxime Pichon

Dear Piotr,

The correct answer will depend of your librairies.

Do you work on amplicons ? Do you work on metagneomics?

The first one will need more than 10% (15% at least) when the second will need 5%.

Best regards.

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Science method

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