We have tried imaging the NMJs of adult drosophila legs before, whereby on anti-HRP was used as the only primary antibody. Several papers that I have read through have also mentioned anti-Dlg and anti-Brp as good candidate antibodies to visualize the boutons with greater precision. However, at the moment, we do not have anti-Dlg and anti-Brp so I am thinking of trying out another antibody to stain. Would anti-vGlut be a good choice of primary antibody to visualize the boutons?
Hi, NanoTag Biotechnologies offer a nanobody that recognizes VGLUT1 (FluoTag-X2 anti-VGlut1) and might be suitable for your application. Since we do not know if it will work in Drosophila, we would like to offer you a free test sample. Shipping in Europe will be on us, however for outside Europe it would be great if you could provide us with your FedEx account number.
More Info: https://nano-tag.com/products/fluotag-x2-anti-vglut1
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