I will perform a MethyLight assay with DNA of brain rats tissue. Wich control should I use to normalize de methylation percent of reaction? We found inventoried controls for humans, but not for rats.
You can use high methylated DNA control (EpigenDx) mixed with genomic DNA (commercially available from Takara) at different ratios. If to differentiate less than 5% differences among samples, high coverage targeted NGS is recommended.
You can use rat sperm DNA as low methylation control and high methylation control from EpigenDx, Inc. If you are working with imprinted genes, cytokines, and other high methylated genes or regions, you can try the EpigenDx low methylation control, too.