What should be the positive and negative control in NGS for sequencing a tumor to detect blank therapies?

10 July 2019 1 7K Report

I´ve been searching for controls in NGS but I´ve only find quality controls. So I´m in doubt if it isn't necessary the positive and negative control during the sequencing.

Hope someone can help me

Balig Panossian

On top of Pritam Kumar Panda

's answer i would like to add that annotating the effect of SNP variants ( say, with SNPEFF ) would give you synonymous/nonsynonymous mutations that you can further analyze to see if they change the amino acids in question.

It would be wise to have a list of the amino acids of the wildtype alleles so that you can compare the mutants.

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