Are Pseudogenes found in all different types of cells (living and non-living) including bacteria?

How to deprive ATP in cancer cell?

I want to increase chromatin compaction of cancer cell and to see the function of interested gene in this process. Is there any method to make it?

19 November 2019 5,972 1 View

Any suggestions for an accurate tool in bioinformatics to find pseudogene?

By giving a sequence as an input, are there any available computational tools for finding the pseudogenes in a particular organism?

05 June 2019 6,591 4 View

Trouble shooting allele specific pcr?

am setting up allele specific pcr for cyp21a2 gene that has a pseudogene. i have already amplified the normal gene with specific primers. using this as secondary product i am trying to set up...

31 December 2018 5,287 4 View

Is there anyone familiar with CYP21A2 gene?

Hello all, I am using panel sequancing and I found a homozygous mutstion in cyp21A2 gene. I did sanger but couldnt find this mutation. I think there is a possibility that I sequanced pseudogene....

09 October 2018 2,831 0 View

Does an alternate sequence in in-silico PCR mean presence of a pseudogene?

I tried designing primers for ELANE gene and came up with the following two sequences in in-silico PCR: >chr19:855442+856209 768 bp size >chr19_KI270868v1_alt:34328+35095 768 bp size. Both...

01 February 2018 9,010 3 View

Does an alternate sequence in in-silico PCR mean presence of a pseudogene?

I tried designing primers for ELANE gene and came up with the following two sequences in in-silico PCR: >chr19:855442+856209 768 bp size >chr19_KI270868v1_alt:34328+35095 768 bp size. Both...

31 January 2018 3,122 3 View

What should I do if my primers are amplifying two equal sequences (a gen and a pseodogene)?

10 November 2017 4,006 4 View

What should I do if my primers are amplifying two equal sequences (a gen and a pseodogene)?

06 November 2017 1,263 4 View

How to identify the Pseudogene in whole genome sequence ?

I have to identify the number of pseudo gene present in genome sequence, Please suggest me what are the methods by which i can identify the pseudogene sequences. Please suggest me some...

05 June 2017 6,777 3 View

Can you use the following methods to investigate these experimental situations?

1) Chromosome Translocation: chromosome microarray + FISH? + CGH? 2) Determination of the levels of gene expression of Gene XYZ with or without hormone treatment in a cell line: FISH +...

04 May 2017 6,208 1 View

Abhijeet Singh

Yes, there are a lot of studies describing the presence and identification of pseudogenes in bacterial genomes. And yes, pseudogenes can be analysed in qPCR with pseudogene specific primers.

Frederic Lepretre

Hi Ali Kazemnia

as Abhijeet Singh mentioned, primers can be designed for gene/pseudogene specific amplification at the only condition to design primers in sequences different from both templates. those differences must be at 3'end of primers. If not possible because differences define long fragment, try to design lonPCR primers and use amplicons as template for second PCR with right length.

fred