Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

How can critical thinking be developed in scientific research?

Dear researchers, As I know, critical thinking is an essential feature that should be in scientist. Can you tell me how this skill can be developed? or how do you gain it from your long research...

16 September 2020 2,608 4 View

Online miRNA target prediction tool and related- down stream pathways?

There are many miRNA target prediction softwares, can you help me from your experience which one is the best? What are the consideratios I must take? because I have no experience in it...

02 September 2020 6,714 5 View

Fault analysis with Simulink- abnormal response after fault clearance?

The attached simple model with SIMULINK after clearing the fault, the Simulink is having abnormal responses. Bus voltage V3 is oscillating. anyone facing that issue?

09 April 2020 2,882 3 View

Can I appeal against a journal editorial decision?

Can I appeal against a journal editorial decision?

28 November 2018 8,076 50 View

Efficient antibodies against Gria1 (GluR1) and/or Grin2a (GluN2a)?

Someone could recommend any industrial antibodies against these glutamate receptors based on self experience? I am just looking for using in Immunoblot or Western.

22 March 2018 2,761 1 View

Isolation of Caulobacter from environmental samples. How?

Using independent-culture approach, I have detected abundant 16S sequences belonging to Caulobacter sp. from agricultural soil samples, but I couldn't be able for isolating it using media like TSA...

07 August 2017 3,572 4 View

Isolation of Caulobacter from environmental samples. How?

Using independent-culture approach, I have detected abundant 16S sequences belonging to Caulobacter sp. from agricultural soil samples, but I couldn't be able for isolating it using media like TSA...

02 August 2017 6,077 3 View

How to convert Ti (integral time) and Td (derivative time) to I and D parameter in function block parameter for PID controller (SIMULINK)?

I need help on my problem. I managed to tune my PID controller using Ziegler-Nichols continuous cycling method and able to determine my Kp, Ti (integral time) and Td (derivative time). But the...

22 May 2017 5,641 8 View

How can you succesfully integrate UDG enzyme digestion in to a LAMP protocol to eliminate carry-over contamination?

I have been trying to use the UDG enzyme in my LAMP protocol but I can't seem to find a way to successfully eliminate the false positive results.

23 January 2017 5,443 1 View

Qiagen Blood & Tissue Kit with plants?

I know Qiagen makes plant specific extraction kits, but has anyone tried the DNeasy blood and tissue kit with plant material? Thanks, Matt

24 March 2019 8,520 2 View

Qiagen Blood & Tissue Kit with plants?

I know Qiagen makes plant specific extraction kits, but has anyone tried the DNeasy blood and tissue kit with plant material? Thanks, Matt

02 March 2019 3,714 2 View

What is Melocactus cephalium growth speed?

Bonjour, I am looking for informations about the growth speed of Melocactus cephalium. Would you have data, references ? seasonnal greetings marc

20 December 2016 4,562 3 View

Cell and chromosome?

I am studying the cell and chromosome of Trichogramma(Hym.: Trichogrammatidae). I used of colchicinfor the cessation of cell division and acetic- orcein for staining of them. I got the images of...

11 December 2016 6,574 9 View

What is the basic difference between telomere and subtelomere?

01 February 2016 4,064 3 View

What is the best definition of cytogenetics and karyology?

What are the differences between cytology, cytogenetics, and karyology? How do they deal with each other?

22 March 2015 2,758 3 View

What is the best definition of cytogenetics and karyology?

What are the differences between cytology, cytogenetics, and karyology? How do they deal with each other?

02 March 2015 4,467 4 View

Can anyone help me to identify this plant?

06 July 2014 1,550 13 View

DNA and RNA extraction from cacti. Any references or suggestions?

I'm trying to do a nucleic acid extraction from cacti. Has anyone worked with that? I'm facing problems to extract DNA and RNA from the aerial part, mainly due to the amount of mucilage. Most of...

07 August 2013 5,042 5 View