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Questions related to Molecular Cell Biology
We are planning to buy shRNA for our experiments. can anyone suggest which form is good like whether one should buy bacterial stocks, plasmid or lentiviral particles? Is it good to buy bacterial...
07 July 2017 10,152 3 View
Does anyone has some suggestions about the isolation of the immune cells, endothelial cells and epithelial cells in lung? I am going to do some q-PCR to measure some gene level in these different...
07 July 2017 9,204 1 View
Is there such an existing kit or maybe electrophysiology experiment ?
07 July 2017 5,255 2 View
Hello!My substance decreases the percentage of cell numbers in the G2/M phase (cell cycle) compared to the saline control in the cytometry assay. What can this suggest?
07 July 2017 7,123 3 View
If a dead bacterial cell still maintains the integrity of cell wall, would it respond to Gram stain?
07 July 2017 9,437 3 View
Is it possible to visualize synthesized cDNA from RNA by gel electrophoresis.
07 July 2017 2,121 4 View
I would like to clone and express a segment of viral RNA in C6/36 mosquito cells but do not know which plasmid to use for the experiment. I tried using a plasmid that I use in mammalian cells with...
07 July 2017 4,400 1 View
Stupid mistakes.... 1) will the UV light affect the doxirubicin stored in a plastic eppendorf tube 2) is the efficency compromised by having it in room temperature for less than 24 hours?
07 July 2017 727 3 View
06 July 2017 3,074 3 View
Now I need to determine the subcellular localization of a given lncRNA with qPCR after cellular fractionation.However,I am not sure whether same amount(e.g.1ug) or same proportion(e.g.1%) of...
27 June 2017 8,333 7 View
Looking for different methods to reduce the metabolic activity of cells. Primarily HepG2 cells, but muscle or adipose tissue cells either.
26 June 2017 3,181 3 View
Hi, I'm trying to generate p53/p16 double knockout cell line. I managed to knockout p53 using Crispr. Now, I can't manage to knockdown/out p16. I have tried so far 5 different Crispr targeting...
23 June 2017 6,876 3 View
Area- plant phylogenomics, phytogeography and molecular phylogenetics?
22 June 2017 4,848 3 View
I need clone a gene from Thermus thermophilus Strain HB8 but have no PCR template. Thanks
15 June 2017 6,681 2 View
Immunofluorescence assay, my target protein locates both in ER and Golgi, I want to calculate the percentage in the two organelle.
15 June 2017 7,703 2 View
Triton x-100 used if we are looking for nuclear protein so, I want to know localization of proteins first that will help me to find the appropriate permeabilization solution.
14 June 2017 8,662 2 View
I used MDa-MB-231 cells, interested in protein that I want to overexpress by using Adenovirus I want to synchronize the cells to do cell cycle analysis Any Suggested steps ? Thanx
13 June 2017 9,564 1 View
Hi to all, Recently I have read some paper related to extracellular vesicles. In the article, I found that some Transcriptional factors(TF) and other macromolecules can be transported by EV. Given...
13 June 2017 1,567 1 View
the protein expresses specifically in brain and lung.
08 June 2017 5,054 4 View
We have detected DNA from a protozoan parasite in lymph nodes of infected mice during 15 days after the infection. Could this DNA be related to live parasites?
06 June 2017 7,905 6 View
I am trying it on fibroblast proliferation. However, small dose of FXIIIA( 1ug/ 500 ul-well) can kill my cells. I have removed thrombin by P-aminobenzamidine agarose and other toxic detergent by...
06 June 2017 4,813 4 View
A colleague of mine recently saw a talk in which the presenter claimed to deliver a membrane-impermeable small molecule metabolite into cultured cells using Lipofectamine (or a similar cationic...
06 June 2017 754 3 View
I got gene expression profiles from single cell sequencing. Some cells have high expression of mbp (mature oligodendrocyte), but low expression of olig1,2, sox10 and Mag (Oligodendrocyte...
06 June 2017 6,604 3 View
06 June 2017 1,859 2 View