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Questions related to Molecular Cell Biology
Hi Anybody was able to use siRNA to block STAT3 alpha specifically? Thanx
17 July 2017 2,088 1 View
I have been trying to block KDM6B and KDM5B with to chemical blockers (subretinal injection-rat). I could not detect any changes in treated eye vs control eye with Western Blotting for H3k27me3...
12 July 2017 3,542 2 View
Hi there, I was wondering if the cells have the "spread-out" spider web looking morphology, does this mean that the cells are senescencing? Do they also have "bubbles" in their cytoplasm? Thank you,
11 July 2017 883 3 View
I know annexin-v is used to identify its presence, but is there a way to actually quantify it and differentiate it from PS inside the cellular membrane? (Also, is there a membrane-impermeable dye...
11 July 2017 4,648 1 View
I always understood fold change calculations following a qPCR to be calculated by 2^-(ddCT). Is this incorrect, my mentor thinks it is? Potentially they are thinking of something else. Could you...
10 July 2017 2,443 5 View
I used an anti-ANT antibody fluorescently labelled with Alexa Fluor red and tried to show co-localization with mitochondria using mitotracker red but it did not work. Why does it not work and what...
09 July 2017 9,417 2 View
Red blood cell is a kind of nucleus free cell, theoretically there should not be mRNA transcription happened in the cytoplasma. But given that the red blood cells can survive in circulation for a...
07 July 2017 2,042 7 View
I've filter LDL in a 0.45um filter but then I don't have any effect when were incubated with the cells. Any suggestion for how pre-saturated the filter because if LDL should be here?
07 July 2017 7,288 1 View
I am looking to immortalize skeletal and cardiac myoblasts without messing with p53. Skeletal myoblasts can be readily transformed with a combination of mTERT and CDK4 (I suspect that this will...
07 July 2017 5,764 4 View
07 July 2017 9,346 1 View
What solution I can use for dilution of condition media and/or washing?
07 July 2017 476 1 View
Ewa L Gregoraszczuk
07 July 2017 7,003 0 View
Are there any well established fluorescent dyes (alternatively to PicoGreen) that can differentiate between supercoiled and relaxed DNA, so that they are able to measure the difference in...
07 July 2017 1,670 3 View
07 July 2017 1,976 2 View
Is it possible to do transformation without using plasmid? Can I directly force DNA fragments from PCR into a bacterium without ligating it into a plasmid? And how do I do it? (I am not using E.coli)
07 July 2017 5,743 10 View
Hi, does anyone know any fungal housekeeping genes + primers? Would it be useful as internal control of PCR? I extract both human & fungal DNA from samples, so housekeeping gene has to be a...
07 July 2017 5,903 0 View
I know that regeneration happens at PNS to some extend. I'd like to know whether injured (completely transected) axons fuse with the distal part of the axon in the PNS after succesful therapies....
07 July 2017 6,541 2 View
I clone the E2 gene (444bp) in pACT2 vector during PCR the insert amplification was occur but during digestion the insert release is not occur ?
07 July 2017 5,455 8 View
UltraRIPA Diagnocine: http://shop.diagnocine.com/UltraRIPA-kit-for-Lipid-Raft-F015.htm
07 July 2017 3,158 1 View
I use FluoZin-3 fluorescent dye to test the transport activity of a membrane transport protein. I need help to separate proteoliposome from the excess fluorescent dye which remain outside. My...
07 July 2017 1,735 0 View
Hello! I am microinjecting Tol2 and UAS:mScarlet into zebrafish, and I was looking up what the optimal concentration of Tol2 and plasmid I should be injecting. There were a couple protocols that...
07 July 2017 9,665 4 View
I am looking for an easily-transfectable cell line whose glycogen content can be easily perturbed by adding, for example, epinephrine or insulin or something. Is there such a thing out there?
07 July 2017 9,408 1 View
analysis of TDT
07 July 2017 9,321 1 View
use for in vivo experiment.
07 July 2017 8,252 2 View