Is it possible to visualize synthesized cDNA from RNA by gel electrophoresis.
Hey,
since cDNA usually derives from mRNA (oligo-dT-Primers) which only makes up about 2-5% of total RNA I would guess that after RNAse treatment you would not see any specific band structure on a agarose gel. When analyzing RNA via electrophoresis you normally see the big rRNA bands that disappear after RNAse treatment.
So generally you need to amplify certain common genes with PCR to determine the success of cDNA synthesis.
Best regards
After getting cDNA
Add terminal transferase with any of the nucleotides (eg dCTP)
It is called homopolymer tailing
Select the sutable vacter do the restriction digestion
Add terminal transferase with dGTP
allow cDNA + Vacter to hybridization
Add the DNApolymerase to fill the gap
Insert in tu the sutable host
Sequence it.......
I am fully agree with Soner Öner-Sieben. I think you can simply verify the cDNA synthesis using Nanodrop spectrophotometer. Then, amplify certain common genes via PCR to determine the success of cDNA synthesis
You can confirm your cDNA by amplifing certain common genes using normal PCR. You can design primers on exon regions and when you amplify with cDNA, it will give different size of band in comparison to reaction performed using DNA as template.
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