Ways to reduce the metabolic activity of cells in-vitro?

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How i can intersect 2 Achses in Amira or 3D Slicer ?

English: Hello, I'm working on a doctoral thesis in the field of the cochlea. I've reached a point where I need to define axes on a segmented cochlea. To do this, two template axes in 3D space...

12 December 2023 6,223 0 View

Segmentation with Amira-Avizo?

Hello, I would like to ask if anyone can help me : I have to segment a cochlea first and later measure the volume using Amira software as part of a PhD thesis. Could someone tell me how to do...

24 September 2023 274 2 View

Imaging immunofluorescence stained histology on Zeiss AXIO with HXP 120 V laser. Unexplainable striping on image due to laser?

Hi all, I'm trying to image fluorescent stained spleen and I seem to be getting some aberrations on the images, a kind of striping effect. I suspect it is from the laser. Channels affected are...

31 January 2023 5,897 3 View

Protease Q: Process human synovial fluid samples for protease analysis and it turns to jelly. Think DNA is the cause, any suggesitons on how to fix?

Processing synovial fluid samples for protease analysis with a mass spectrometer. Samples are centrifuged at 4 degrees 2200g for 15 min, then heat-denatured at 95 degrees for 10 minutes. Samples...

23 January 2023 2,945 3 View

Bacteiral protease activity using fluorescence tagged peptides?

I am trying to calculate the amount of peptide I would need to order to complete a bacterial protease activity assay using fluorescence. I hoping to look at E. coli and S. aureus strains with...

09 January 2023 9,934 2 View

Have I set this up correctly to determine sample size using G*Power?

I am working on my first educational design research study and trying to determine what the minimum sample size should be. This is a pretest posttest model where the same students take a...

16 December 2022 9,548 1 View

How can I determine genetic interaction based on Arabidopsis root growth rate measurements?

I have a series of root growth rate measurements of Arabidopsis lines carrying a single KO in gene x or gene y, and a series of measurements of lines carrying a double KO in both gene x and y. I...

03 November 2022 5,868 0 View

How do I perform statistical tests on multiple response sets in SPSS?

I have conducted an online survey and I want to run a statistical test to determine of there is any correlation between, for example: 1. The number of years of experience a person has (selected...

05 June 2022 9,284 2 View

Is thawing and re-freezing protein samples a necessary evil or is it best to aliquot 3> times?

Hello, I have 100 tissue samples (50 cortex and 50 hippocampus) that have been homogenized (sigma RIPA + sigma protease inhibitor (PI)). I have made three supernatant aliquots per sample. I...

05 June 2022 8,092 0 View

How do you download ADCP data in csv or ASCII format using Pulse?

We only seem to be able to download .ENS files in section "Download Data" in Pulse, but we would like to have csv or ASCII.

19 October 2021 1,364 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Gordon L Warren

Temperature would seem to be the most obvious way. Q10 temperature coefficients for most enzymatic reactions are in the vicinity of 2 meaning that for a 10 oC decrease in temperature, the cells' metabolic rate would decrease by half.

Kiera Ward

Thank you very much Gordon!

Delilah MH

you could try lowering the serum concentration in the media, as it contains cholesterol