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Questions related to Molecular Cell Biology
I am at a small primarily undergraduate institution with limited resources, but we are eager to establish some important and feasible molecular/cell biology experiments on questions related to...
03 March 2019 5,682 2 View
I am going to do MTT assay on HK-2 (adherent cell line) cells? I have checked many different protocols online but they are so diverse and now I am not sure which one will be suitable for my cell...
03 March 2019 2,605 3 View
What do you know about the comet assay kits? Are they useful? How profitable are they? Is it worth buying? How was your experience with them?
03 March 2019 8,824 2 View
Hi everyone, I am newbie on using the confocal and I am wondering if the setting on all the images I take should be the same? in order to compare between them? because when I took the images I...
23 February 2019 1,940 3 View
If we have an unknown cell, for basic studies on its properties, we need to detect the combination of channels on the cell.except voltage clamp method,how we can do that? for example If we have a...
21 February 2019 9,288 4 View
I found this interesting device (https://darwin-microfluidics.com/collections/microfluidic-organ-on-a-chip/products/ddi-chip-distance-dependent-interaction-chip-pack-of-3). Have you any experience...
02 February 2019 6,013 4 View
There are a big mystery in the world of molecular biology regarding the part of genome which must be present but cannot be found.
02 February 2019 9,184 11 View
I am trying to calcium-image marine invertebrate larvae, by incubating larvae in FSW in AM-ester dye (Fluo-4 AM). I am wondering if dye saturation is the reason I'm not observing any signal...
25 January 2019 3,283 2 View
01 January 2019 649 2 View
In a pinch, can 70% methanol replace 70% ethanol for sanitizing surfaces prior to cell culture?
01 January 2019 3,325 13 View
can we pool cancerous serum to be used in cell culture instead of FBS or normal human serum ?
01 January 2019 2,592 7 View
Dear researcher Most MD studies are performed for extracellular conditions where NaCl concentration is high, while we are trying to perform MD for some intracellular molecule, where K, Mg, HPO4...
01 January 2019 5,117 1 View
Hi All, Has anybody with experience in inducing mitosis by any chemical reagents..? My main aim is to activate APC/cdc20 complex along with the start of M phase. Currently only method I am...
12 December 2018 7,773 4 View
Is it possible to identify E.coli strains using PCR-based methods? How does the identification of E.coli strains work? Is it based on 16srRNA and phylogeny study or ...?
12 December 2018 6,095 4 View
Hi, I'm utilizing FDC-P1 cells (ATCC® CRL-12103™) in my experiments. Now, I'm in a phase that I'm going to perform rt-PCR using them. For this I need information about the average total RNA yield...
16 November 2018 6,311 2 View
Hi! Some of the lentiviral transfer plasmids I'm using have this short form of the CMV promoter that is 350bp instead of 589bp for the usual CMV promoter. Does this short form have a name?...
11 November 2018 5,133 2 View
Sequencing of human genome at the center of interest in the biomedical field over the past several decades and is now leading toward an era of personalized medicine. During this time, DNA...
11 November 2018 3,102 3 View
Hello, I wonder is it possible to store the cells (Plate) at -80 degrees Celsius for later protein sampling (western blot)? I want your opinions
11 November 2018 3,914 7 View
11 November 2018 8,930 3 View
I want to work on PBS in molecular breeding and want to know are their any genes or tightly linked markers available for PBS in Corn. I want use these in our breeding programme.
11 November 2018 1,537 2 View
Membrane proteins can be inserted into membranes via different mechanisms and in different orientations. I am specifically interested in mammalian cells (not bacteria or yeast). Does anyone know...
11 November 2018 2,069 2 View
02 November 2018 2,958 6 View
Hello community, we currently working on an automatic algorithm for erytrocyte detection in stereo-microscopy images for cell counting. Additionally we want to determine the percentile amount of...
24 October 2018 3,424 2 View
Free radicals are highly reactive. They can start a chain reaction which is extremely dangerous when they react with important cellular components such as DNA. Despite all said above, what makes...
18 October 2018 8,305 3 View