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Questions related to Molecular Cell Biology
This is a rhetorical question, as I´ll give an answer. I get asked questions through research gate messaging from time to time or I see questions on RG where I feel that I have the know how and...
09 September 2017 8,296 13 View
Although Trypsin causes cellular damage and time of exposure should be kept to a minimum, in protocols it is suggested that further actions can be made on cells without washing them with PBS. the...
09 September 2017 8,101 9 View
I am measuring a sample of histone octamer which is in buffer containing 20mM Tris-HCl, 1mM EDTA, 2M KCl, 10mM DTT, 0.5mM Benzamidine and MilliQ water. I have prepared 1M Benzamidine in Ethanol...
09 September 2017 5,996 8 View
Does anyone have experiences in treatment of samples (from food or pure culture) by DNAse to digest free DNA prior to DNA-extraction? The free DNA shall not be amplified by real-time PCR and will...
09 September 2017 5,395 7 View
I know benzene rings in tryptophan at 280nm give the absorption so is this the only criterion for a molecule to possess a chromophore?. Or do the functional groups play a pivotal role along with...
30 August 2017 3,167 5 View
hi i am not sure if it is alright to analyse my data this way. So i prepared 3 samples of controls, 3 samples of condition at 1hr exposure, 3 samples of condition at 6hr of exposure. i collected...
15 August 2017 8,704 4 View
08 August 2017 395 6 View
08 August 2017 7,836 5 View
Hello colleagues, We are currently working on the MuLE system that was published on JCI (J Clin Invest. 2015;125(4):1603–1619. doi:10.1172/JCI79743.) . However, I'm having some technical...
08 August 2017 2,442 5 View
I am looking forward to a method that can delay/inhibit C2C12 differentiation.
08 August 2017 7,611 3 View
Let us say a bacteria has a generation time of 20 minutes. How do we find the different promoters which are active at the 15th minute (for example)?
08 August 2017 1,182 2 View
Hi Hollow DNA spheres. Im not getting a good number to stick to mica for imaging and they seem to be aggregating when I try and get a size distribution of them in a zetasizer. Im trying to use a...
08 August 2017 3,319 2 View
simply.
08 August 2017 7,799 13 View
Along with which virulence factors produced during that biofilm transition stage from the planktonic cells. Its good that it was found that cells experience shear stress and flow for formation of...
08 August 2017 4,035 6 View
05 August 2017 9,325 4 View
DNAase II, inhibitor, viability
26 July 2017 9,769 2 View
I've filter LDL in a 0.45um filter but then I don't have any effect when were incubated with the cells. Any suggestion for how pre-saturated the filter because if LDL should be here?
18 July 2017 791 1 View
Is it possible to do transformation without using plasmid? Can I directly force DNA fragments from PCR into a bacterium without ligating it into a plasmid? And how do I do it? (I am not using E.coli)
18 July 2017 7,156 8 View
I am looking to immortalize skeletal and cardiac myoblasts without messing with p53. Skeletal myoblasts can be readily transformed with a combination of mTERT and CDK4 (I suspect that this will...
17 July 2017 8,220 3 View
Hi Anybody was able to use siRNA to block STAT3 alpha specifically? Thanx
17 July 2017 1,989 1 View
I have been trying to block KDM6B and KDM5B with to chemical blockers (subretinal injection-rat). I could not detect any changes in treated eye vs control eye with Western Blotting for H3k27me3...
12 July 2017 3,482 2 View
Hi there, I was wondering if the cells have the "spread-out" spider web looking morphology, does this mean that the cells are senescencing? Do they also have "bubbles" in their cytoplasm? Thank you,
11 July 2017 789 3 View
I know annexin-v is used to identify its presence, but is there a way to actually quantify it and differentiate it from PS inside the cellular membrane? (Also, is there a membrane-impermeable dye...
11 July 2017 4,559 1 View
I always understood fold change calculations following a qPCR to be calculated by 2^-(ddCT). Is this incorrect, my mentor thinks it is? Potentially they are thinking of something else. Could you...
10 July 2017 2,359 5 View