Triton x-100 used if we are looking for nuclear protein
so, I want to know localization of proteins first that will help me to find the appropriate permeabilization solution.
Hi,
this might help:
https://elifesciences.org/articles/16950
Best regards,
Koraljka
Hi Mahmoud, this sounds like a macromolecular Heisenberg principle. You want to know where the protein is before you attempt to detect it.
My recommendation is to permeabilize and fix the cells using digitonin and PFA:
http://www.nature.com/protocolexchange/protocols/1994
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