How could I know the location of proteins to find best method for Immunofluorescence staining especially when we use Triton?

More Mahmoud Mo's questions See All

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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How much Yeast should we add to fermentation media?

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Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

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Trying to make optimization for Mixer on different pdk ?

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Is it good choice to use the SARC/J auxiliary basis set with def2-SVPD basis set without including any relativistic effects like ZORA or whatever?

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Do the methods of calculating the absorption coefficient (Alpha) from absorbance differ between powder samples and thin films?how is it calculated ?

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How do quick initial-test to select antibiotic-producing fungal strains?

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Does anyone have detailed methods and instructions on how to count the numbers of intraepidermal nerve fibers (IENFs)/mm using Fiji - ImgaeJ?

Counting the numbers of intraepidermal nerve fibers

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Effects of Aeromonas caviae and Aeromonas veronii on Freshwater Fish?

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What is the solution to this problem in the Abaqus program? Abaqus ?

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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Could it be a cell culture contamination?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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Can I use a HisTRAP column for affinity chromatography?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

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Why does my protein refolded to beta sheet during thermal denaturation analysis?

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Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

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Weak DAPI staining after immunohistochemistry - how to improve?

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The Curse of Evolution and Complexity?

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Koraljka Husnjak

Hi,

this might help:

https://elifesciences.org/articles/16950

Best regards,

Koraljka

Steingrimur Stefansson

Hi Mahmoud, this sounds like a macromolecular Heisenberg principle. You want to know where the protein is before you attempt to detect it.

My recommendation is to permeabilize and fix the cells using digitonin and PFA:

http://www.nature.com/protocolexchange/protocols/1994