What is the best way to detect de novo calcium accumulation after i.v. injection of calcium (CaCl2 + Ca45 as tracer) in rodent organs?

Rodents will receive calcium injection to specify preferred tissue distribution after my drug treatment. Measuring radioactivity from blood sample is fairly easy, is it feasible to determine...

09 October 2018 7,481 5 View

Which staining would you recommend to stain chondrocytic cells (connective tissue, collagen?) bone cells (osteoblast, osteocasts, osteocytes) ?

I will have bone tissue embedded on the microscope slide/parafin AND vascular tissue where chondrocytic cells/bone cells might be present as a atherosclerotic plaque. I was wondering whether...

10 November 2017 9,134 4 View

What live/dead cell assay would you recommend for cells encapsulated in alginate gel system?

02 March 2017 1,604 0 View

How many smooth muscle cells should I seed per well in 48-well plate along with alginate gell encapsulation?

Thanks!

02 March 2017 5,237 0 View

Whats the optimum concentration of NMDA or glutamate to activate NMDA receptor expressed in HEK293 cells ?

04 May 2016 314 5 View

What is the optimum percentage of SDS-PAGE for proteins of mass 34kDa and 175kDa when both need to be loaded on one gel ?

I am trying to detect proteins using Western Blot Technique. Due to the size difference, smaller protein of 36kDa was not seen on the blot read-out. Would gradient gel be a better idea to detect...

31 December 2015 4,194 10 View

Has anyone grown VSMC's (vascular smooth muscle cells) in alginate?

01 January 1970 5,965 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View