Does Gram stain work with dead bacterial cells?

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Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

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Katie A Burnette

All bacteria are killed during the Gram staining process by either heat or methanol fixation to the slide. So, yes it does work on dead cells.

Vijayata Singh

Katie is right. Well there is kit which can distinguish using this protocol between live and dead bacteria 10.1186/s12866-015-0376-x.

Martin Klvana

Is it really necessary to kill a living thing in order to find out what it is (was)?