If a dead bacterial cell still maintains the integrity of cell wall, would it respond to Gram stain?
All bacteria are killed during the Gram staining process by either heat or methanol fixation to the slide. So, yes it does work on dead cells.
Katie is right. Well there is kit which can distinguish using this protocol between live and dead bacteria 10.1186/s12866-015-0376-x.
Is it really necessary to kill a living thing in order to find out what it is (was)?
24 February 2021 1,005 1 View
Except ( Tomato-Brinjal-Capscum-Chilli) .
27 December 2020 8,362 12 View
In Bundelkhand region of Central India, Asphodelous tenuipholius is major problamatic weeds on farmers field. Any suggestions from weed scientists
29 November 2020 1,207 4 View
We doubt that some people add urea in fish based food item, any one can suggest some method by which we can detect the uear qualitative and quantitativly.
16 November 2020 6,839 3 View
I am working on phase solubility studies between beta-cyclodextrin and drug? I used more concentration of beta-cyclodextrin till 30mM despite its maximum solubility of 16mM. Usually, above maximum...
30 October 2020 3,921 9 View
I have 3 drugs mixed in a solution coming out from a microfluidic device. I know the concentration of each drugs at inlet. If I have absorbance data of each inlet drug solutions, how to estimate...
13 October 2020 898 3 View
03 October 2020 3,834 3 View
Hi, Our lab started using tdTom mice for lineage tracing. Currently we are using 2 antibodies to detect tdTom one from Clontech and other from rockland. When we use tdTom antibody for...
29 August 2020 4,105 2 View
I have designed an antenna to operate at 14.25 GHz( 14 to 14.5 GHz)uplink frequency of Ku band with linear polarization. what kind of application is suited for my designed antenna? and reference...
11 July 2020 699 2 View
Sir Iam doing MATLAB SIMULINK implementation of Switched capacitor multilevel inverter with high and low switching frequency technique.so i need help in doing matlab coding for optimizing SHE and...
23 June 2020 7,407 2 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
02 March 2021 3,060 3 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View