Does anyone have the Thermus thermophilus Strain HB8 or its genomic DNA?

More Shouqiang Cheng's questions See All

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View

Clarification on 3 x 2 factorial designs and reporting?

Hi, I am currently looking at a study where they used a 3 x 2 factorial design where they had 3 diets (A, B, C) and 2 enzyme supplementation levels (0 vs 1 %). Thus giving 6 treatments. Authors...

26 May 2024 4,626 1 View

Factorial Design Experiment Data in a Meta-Analysis?

Hi, I am currently creating a meta-analysis on the effect of growth performance of pigs in response to dietary fiber content in experimental diets as well as the influence of a fiber enzyme...

25 May 2024 8,855 1 View

Gold Cleaning Process before Parylene Coating?

I need to run CVD parylene deposition onto gold wires. How can I clean the gold surface before coating to achieve good adhesion? Thanks a lot!

07 April 2024 4,537 3 View

RNA QC for RNASeq - Proceed or not proceed?

Hi All, I would like to seek for your expert input for below queries on RNA QC for RNASeq: Can we proceed for RNASeq run on RNA less than 10ng, with DV200 in between 68 - 80%?Details as below:...

18 March 2024 5,936 2 View

What is the difference in natural seismic imaging results between island arc or basalt crystal base and ancient craton crystal base?

What is the difference between island arc or basalt crystal base and ancient craton crystal base in natural seismic imaging or seismic wave speed?

16 February 2024 314 0 View

Mplus - moderated mediation - covariances among paths?

Hi! I am trying to analyze a moderated mediation using mplus. I first started with a simple mediation, so X -> M -> Y. Path a (X -> M) is not significant, while Path b (M -> Y) is...

25 January 2024 3,738 2 View

How to model hysteresis property of soil in abaqus?

Hello, I want to simulate the hysteresis characteristics of soil under cyclic loads or earthquakes. I would like to know if there are methods other than using UMAT to write a constitutive law. For...

17 January 2024 2,754 0 View

What role does atomic diffusion play in the grain growth process of a metallic material?

In metallic materials, the process of grain growth is also the process of grain boundary migration. So what role does atomic diffusion play in this process? Is there long-range diffusion migrating...

07 January 2024 8,375 2 View

Could you upload full text ?

Is there someone can download 'Targeted ADAMTS13 Replacement Therapy for Thrombotic Thrombocytopenic Purpura' DOI:https://doi.org/10.1016/j.jtha.2023.11.030 full text?

01 January 2024 2,878 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View