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Questions related to Molecular Cell Biology
Is there an option to have non-coloured outputs?
06 June 2018 1,341 0 View
Dear All, I have a ~2300 bp insert with Snabl and NotI respectively( 5 prime to 3 prime). I need to use pPIC9 expression vector which is 8 kb in size. I co digested pPIC9 with SnabI and NotI,...
28 May 2018 3,766 3 View
In a situation where a myelinated neuron (at any location in the nervous system) gets demyelinated, what are the changes that occur in the neuron. May be there are three phasic changes....
24 May 2018 1,545 8 View
05 May 2018 7,468 3 View
Dear researchers I want to know polyubiquitin roles in DNA repair .(briefly)
05 May 2018 8,619 0 View
I have a phlyogenetic tree (see attachment). In the legend, it is written that the distance scale represents the number of differences between the sequences. My question is how can I...
05 May 2018 9,607 10 View
05 May 2018 4,242 9 View
I have cloned different type of sequence(non canonical) after t7 promoter followed by a reporter gene.i am using in-vitro/transcription translation for quantifying reporter. can someone provide me...
05 May 2018 752 3 View
Reactions are carried out in 50 mM Tris–HCl, pH 7.5, 5 mM MgCl2 and 100 mM NaCl using 2 µM cysteine desulfurase (Nfs) in presence or not of 2 µM SufE1. BSA 2 µM can be used as control. In addition...
05 May 2018 3,183 4 View
Hi Everybody, I have senior project about Biosynthesis of Nickel nano-particles via soil Bacteria, I want to know what is the mechanism or Bio-process that is used here? can anyone help Thanks a...
05 May 2018 4,591 0 View
The concentration must be mg/ml concentration or µg/ml? If it is mg/ml, then justify the reason for choosing that high concentration.
30 April 2018 5,823 3 View
I am searching for a protein (or transcription factor) that can interact with a short DNA sequence (that is NOT promoter or some part of promoter for another gene) and this interaction results in...
19 April 2018 5,102 2 View
Hi everyone, I want to use the amplex red assay to quantify H2O2 levels in different cellular clones. Many protocols I found online or previous answers on RG contradict each other. The major...
13 April 2018 8,987 4 View
This is not for microscopy studies. This is for an in cell protein foot printing technique, Fast Photochemical Oxidation of Proteins FPOP. I just need guidance on how to get the unlabeled...
04 April 2018 439 2 View
04 April 2018 8,970 3 View
I've been making qPCRs with SYBR Green and in some of my reactions the efficiency is under 90% (between 80%-89%) Can i use the ΔCt method to analyse my results? If not, what should i do?
04 April 2018 4,772 5 View
04 April 2018 1,317 2 View
It is well known that genes play an important role in controlling all biological activity in our life. Most of these are functional can either in gene expression or in the synthesis if protein......
04 April 2018 2,849 8 View
Hello , Can Anyone explain the differences ? Thanks in advance!
04 April 2018 5,328 2 View
The diffence between the two is the sequence information that is encoded. The tracrRNA or trans-activating crRNA is made of up of a longer stretch of bases that are constant and provide the “stem...
29 March 2018 2,969 3 View
I'm cultivating 3T3-F442A murine pre-adipocytes for an experiment. They suddenly started growing slowly and showed abnormal morphology. I suspected of mycoplasm and as I don't have a kit for...
07 March 2018 8,216 7 View
In many articles and chapters,primary concentrate was on cell impedance measurment but i can not find articles that focus on cell and ECM impedance measurment.
06 March 2018 6,152 3 View
Zoonotic pathogens are widely distributed. The pathogenicity of them was ranged from low to moderate and severe.
03 March 2018 8,757 0 View
DNA profile is so important for each one to do. This step is necessary for forensic DNA purposes
03 March 2018 5,713 0 View