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Questions related to Molecular Cell Biology
How we can diagnosis between DNA hypermethylation and hypomethylation in human
10 October 2018 2,907 5 View
Hello community, we currently working on an automatic algorithm for erytrocyte detection in stereo-microscopy images for cell counting. Additionally we want to determine the percentile amount of...
10 October 2018 1,161 2 View
Is it harder for Thicker tissues to nourish itself or other itselfs mission?
10 October 2018 7,907 1 View
I'm going to follow Inoue's protocol for competent E. coli cells. It says that I should incubate it in 250ml SOB medium 18°C-22°C at moderate shaking. Then, I should read the OD600 in the next...
10 October 2018 8,076 9 View
08 October 2018 5,184 8 View
Everytime i found different OD data which was fluctuating and sometimes continue increase of OD .Though growth curve and colony under microscope dont tell wheather it is contaminated or not.If...
09 September 2018 607 15 View
Chromatin is a complex of macromolecules found in cells, consisting of DNA while A chromatid is one of two identical halves of a replicated chromosome what is the difference between chromatin and...
09 September 2018 5,160 18 View
I usually calculate using the formula c1v1=c2v2 but have observed other people calculate differently. It confuses me. For example, if the number of cells to be seeded is 1x10^4 and the stock is...
09 September 2018 4,390 9 View
I am a fresh man in cell molecular biology. I was studying the liver metabolites function and the inflammatory response with exogenous toxicant. I knew the hepatocyte is the metabolite center for...
08 August 2018 9,752 7 View
Kindly share buffer recipes. Thanks in advance.
08 August 2018 2,041 3 View
Hi, I would like to perform gene disruption in Lactobacillus paracasei. Could you provide a protocol or suggest a grup which is able to perform this kind of analysis? Thank you
08 August 2018 4,516 1 View
Probiotics are good bacteria that are either the same as or very similar to the bacteria that are already in your body. Your lower digestive tract alone teems with a complex and diverse community...
08 August 2018 7,399 8 View
CRISPR/Cas9 is a system found in bacteria and involved in immune defence. Bacteria use CRISPR/Cas9 to cut up the DNA of invading bacterial viruses that might otherwise kill them. Today we’ve...
08 August 2018 6,869 8 View
I am wondering can intracellular metabolic compounds enter the cell from the extracellular? Which mechanisms are involved allow above compounds enter cells? Passive fusion? Cell endocytosis? Or...
08 August 2018 8,099 2 View
07 August 2018 6,267 2 View
I have a set of cells double stained in this manner: first the cells has been live-stained to visualize the protein of interest at the membrane (named: SURFACE). Then, cells have been fixed and...
24 July 2018 9,180 6 View
Hi, I extracted RNA today via the trizol method. My 260/280 ratio is 1.95 while 260/230 is 1.44. I washed the pellet using isopropanol and ethanol. The cells grown and RNA extracted from were...
14 July 2018 2,752 6 View
07 July 2018 7,219 7 View
For optimization of PCR, we need to use accurate and micro-quantities of PCR reagents to obtain reproducible results. In some circumstances when a ready to use pre-mix PCR tubes kit is not...
07 July 2018 3,840 0 View
Hi, I was involved in some projects in memory and learning processes and molecular mechanism of those processes. Now, I want to be involved in any research deals with molecular biology and...
07 July 2018 3,066 0 View
I wish to check antiinflamatory activity of plants on this cell line.
06 June 2018 5,491 0 View
Hi folks, I was wondering what determines a protein to go into the regulated vesicular exocytosis. I am also interested in understanding how vesicles from the regulated pathway are arrested in...
06 June 2018 7,722 0 View
Hello everyone. I want to do DNMT activity analysis by using prostate cancer cells. I want to know whether we can store the nuclear lysate for 2-3 days in -20 degree before performing activity...
06 June 2018 6,587 7 View
I have been trying to make this A. tumefaciens c58ci from last year glycerol stocks stored at -80C competent.I firstly grew in LB medium supplemented with rifampicin and gentamicin. One thing I...
06 June 2018 6,324 3 View