Stupid mistakes....
1) will the UV light affect the doxirubicin stored in a plastic eppendorf tube
2) is the efficency compromised by having it in room temperature for less than 24 hours?
I do not think so
My response is because plastic should not be able to leave UV light pas through.
But nothing is 100% sure in Science: type of plastic? wavelength of UV light ....
I strongly disagree with the first poster. UV light would be deleterious to doxirubicin, per "The inactivation of doxorubicin by long ultraviolet light."
https://www.ncbi.nlm.nih.gov/pubmed/9332465
Hi Liu, I assume that you have not enough of the compound to prepare it again. But I stick to my answer with the reserves due to the wavelength/type of plastic.
Hi Craig, it is not a matter of agreeing (in Science I mean)
After reading the paper you mention I am pretty sure that the compound in a closed Eppendorf plastic tube will not be affected . These sentences are copied form the paper
Methods (note that irradiation is done in well plates WITHOUT THE LID and the type and number of lamps)
Long ultraviolet light exposure
Various concentrations of DOX were dissolved in RPMI medium 1640 or other solutions as indicated below. Drug solutions, 0.2 ml/ well in 24-well culture clusters without the lid, were irradiated in a laminar ̄ow hood with three Blacklight Blue 40-W lamps (Vilber Lourmat, Marne la Valle e, France).
Results (note that RMPI medium is needed for decay)
Irradiating DOX in PBS for up to 30 min did not a ect the growth-inhibitory e ect of the drug, but irradiating it in RPMI medium 1640 resulted in a decrease of the drug activity that was proportional to the length of irradiation. When RPMI medium 1640 was irradiated prior to adding the drug, a reduced growth-inhibitory e ect was not observed (data not shown). These ®nd- ings suggested that if the UVA activated one or more component(s) of the RPMI medium 1640 that could inactivate the drug, this component decayed rapidly and therefore did not a ect the DOX that was added later.
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