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Questions related to Molecular Cell Biology
Hello friends I want to know exactly about the cell cycle so I can do something about mathematical modelling. I know a whole thing about it, but I want to know the processes that occur between the...
03 March 2018 6,343 0 View
in the sequence of my gene, there are two ATG. one at the start of sequence and one in the mid region of gene. so now i am confused which one will act as start codon. is it possible that this gene...
03 March 2018 10,069 4 View
The oncogenic potential of the high risk HPV types lies in the oncoproteins early (E6 and E7) which can bind to and modulate a number of different gene products, in particular, the tumor...
03 March 2018 3,963 9 View
hi i need advise on the matter i performed RNA-seq after exposing cells to mechanical stress. I had designed a device to deliver this stress to cells. Looking at the RNA-seq data, i see some...
03 March 2018 5,382 8 View
Can anybody help me to understand about gene drive? What is the connection of this technology with CRISPR technology? Could it be applicated in plant genome system?
03 March 2018 1,096 6 View
Oncogene have an operator gene and tumor suppressor gene. The tumor suppressor gene is always turn-on and an operator gene is turn-off. There are several factors to create tumor suppressor to turn...
03 March 2018 2,291 12 View
The diffence between the two is the sequence information that is encoded. The tracrRNA or trans-activating crRNA is made of up of a longer stretch of bases that are constant and provide the “stem...
03 March 2018 7,837 6 View
I want to know about the exact processes in any phase of cell cycle, can you give me some sources?
03 March 2018 4,203 0 View
The LncRNA I am interested in studying has many transcript variants, 14 as per ensembl. I want to study the effect of over-expression of this LncRNA. To do so I want to clone the LncRNA into a...
26 February 2018 1,633 5 View
Some of the biochemical processes in our body produce singlet oxygen. This can react with the other substances in body.
02 February 2018 3,630 3 View
A phage in a lytic cycle converts an infected cell into a phage factory...
02 February 2018 9,388 3 View
contemporary cloning vs gene cloning
02 February 2018 9,971 0 View
CRISPR
02 February 2018 9,329 0 View
A genetically modified organism (GMO) is any organism whose genetic material has been altered using genetic engineering techniques (i.e., a genetically engineered organism).
02 February 2018 3,165 12 View
I want to use tetracycline as selection marker for my next experiments. I was advised to perform the experiments in dark. I want to know if this precaution is really required. I haven't found any...
02 February 2018 4,419 7 View
02 February 2018 5,239 6 View
Hair growth
02 February 2018 247 0 View
Some animals can recreate their lost limbs again like how a lizard can recreate its own tail,what if we can inspire this and use it for a disabled person.
02 February 2018 387 0 View
Genomic signature can be intended for many different purposes and applied at multiple different resolutions.
02 February 2018 8,555 1 View
Hello! I am trying to do a ChIP experiment using the IgG sepharose beads from GE Healthcare. I work with a recombinant protein in Arabidopsis fused to TapTag (Calmodulin binding peptide,...
12 January 2018 3,234 2 View
I'm currently planning an experiment for knocking out PARP1 in a human cell line with either the Nuclease or Double Nickase system. I'm not sure which region of the gene would be the best to...
10 January 2018 7,186 4 View
Are Collagen (I/III) and TGF-β right and adequate? I would be grateful if you could give me anything about this topic.
01 January 2018 7,612 3 View
Hi, I am doing starvation assays with Leishmania parasites for which I need to culture parasites in PBS for about 6 hrs. Typically I find that without serum supplement these cells become very...
01 January 2018 2,179 3 View
We use Falcon 96-well assay plates for Saccharomyces cerevisiae growth cuves measuring OD600 under certain chemical conditions. Does anyone know if these plates can be cleaned, autoclaved, and...
01 January 2018 9,180 11 View