Contact experts in Molecular Cell Biology to get answers
2,254 views 1,310 posts
Questions related to Molecular Cell Biology
Hello! I am trying to do a ChIP experiment using the IgG sepharose beads from GE Healthcare. I work with a recombinant protein in Arabidopsis fused to TapTag (Calmodulin binding peptide,...
12 January 2018 3,250 2 View
I'm currently planning an experiment for knocking out PARP1 in a human cell line with either the Nuclease or Double Nickase system. I'm not sure which region of the gene would be the best to...
10 January 2018 7,203 4 View
Are Collagen (I/III) and TGF-β right and adequate? I would be grateful if you could give me anything about this topic.
01 January 2018 7,628 3 View
Hi, I am doing starvation assays with Leishmania parasites for which I need to culture parasites in PBS for about 6 hrs. Typically I find that without serum supplement these cells become very...
01 January 2018 2,200 3 View
We use Falcon 96-well assay plates for Saccharomyces cerevisiae growth cuves measuring OD600 under certain chemical conditions. Does anyone know if these plates can be cleaned, autoclaved, and...
01 January 2018 9,195 11 View
01 January 2018 4,131 2 View
Hi everyone, I want to order this plasmid (https://www.addgene.org/78311/) to Addgene, but when I review it, I can not find the promoter region of ampicillin resistance, so I deduce that the...
01 January 2018 1,472 8 View
01 January 2018 909 4 View
How is proliferation assessed by manual cell counting? I will be happy if anyone can provide protocol on this. I have been told this method is much useful than indirect assays like CFSE but i...
12 December 2017 2,615 0 View
hi i really need advise on which proliferation assay to choose from. my experiment involve growing cancer cells on transwell insert with pores of 0.4um that does not allow invasion. After...
12 December 2017 9,741 0 View
Hi i borrowed the turbo dna free kit (AM1907) from another lab. This is the first time i am using it. To people who had used this kit before, is the tube containing the fluid for the inactivation...
12 December 2017 3,975 1 View
I am going to treat the myocardial infarction rats with conditioned medium for 8 weeks/56 days. It has often been administrated via tail over a short period of time. However, it seems that the...
12 December 2017 2,802 4 View
Have you seen any difference in Taqman system and Sybrgreen RTPCR? Former is way expensive?
12 December 2017 5,641 0 View
After I have trypsinized mammalian cells and removed them from a flask, can I reuse that flask to seed the same culture? This is assuming that I used good aseptic technique. I mainly want to do...
12 December 2017 3,583 8 View
I would like to attach DNA to AFM tip and image it using SEM to check if coating was done properly. I couldn't find any papers that show SEM images of DNA. I would like to know the protocol to do...
13 November 2017 4,331 2 View
If you can explain the misc_feature clearly instead of giving the definition, will be more useful for me to understand. Thank you
11 November 2017 2,124 1 View
I am doing a cell survival assay on cultured stromal cells (obtained from primary culture). How long does the average stromal cell live without serum (FCS/similar nutrition)? I intend to put some...
11 November 2017 1,817 5 View
I kept 10 mL vial in ice bucket in cold room (2-8) maintaining 4 to 5 deg C. it thawed properly. Aliquotes (1mL) of that vial i kept back in -20. When i need one aliquot and i thawed in similar...
11 November 2017 1,790 1 View
10 November 2017 5,818 5 View
01 November 2017 9,635 1 View
Well, let's think that I have a protein that will be transfered into ER and its signal peptide is on N terminus of the protein. I want to cleave the sequence and add it on the C-terminus. Where...
23 October 2017 6,572 2 View
How do I choose the right strain of E. coli for the donor and recipient? Which genes need to be included on the conjugative plasmid besides an antibiotic selection marker? How does this change for...
14 October 2017 9,070 2 View
Which method would detect smaller changes in protein synthesis in a cell?
10 October 2017 6,083 0 View
I am going to do ms pcr but I have doubt on water milliQ water because previously RT pcr result was not coming by using that water. So I borrowed water from another department but that water was...
10 October 2017 3,987 4 View