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Questions related to Molecular Cell Biology
I am working with some strains of cyanobacteria isolated from Zimbabwe's Manyame catchment - I want to extract their genomic material so that I may use it to confirm their identity. In my lab I...
10 October 2019 2,645 3 View
It's not like this cell needs all of the genes in all chromosomes. It needs a lot of energy !
10 October 2019 1,195 3 View
Any good resources here would be helpful, I am specifically wondering about fungal ITS copy number within ascomycetes. I keep reading that ITS has a high copy number however ranges of the copy...
09 September 2019 7,218 1 View
Dear colleagues What are the benefits of Snapgene? How can I get it? Regards;
08 August 2019 7,405 3 View
Recently I have started working on molecular cell biology. Here, I have to perform different types of optical microscopy (DeltaVision for example). I want to understand the mechanisms of these...
25 July 2019 1,973 8 View
07 July 2019 5,906 9 View
We are trying to find the biological correlation between miRNA and certain genes. We found the general correlation through IPA (Ingenuity Pathway Analysis). Thank you for your inputs!
17 June 2019 4,173 5 View
It is confusing concept in the literature that some papers use the term "HER2 positive" and some "HER2 overexpression". You may refer to the definition in the specific paper, but sometimes they...
05 May 2019 4,981 3 View
Recently become quite interested in the lifecyle and molecular bio of chlamydia. Is it right that the chlamydia gets into host cell through endocytosis and live within phagosome? Have read paper...
05 May 2019 7,840 4 View
Could you imagine to create a video explaining the essential of you publications yourself? Why would you want to create a video for you publication? Did you produce these kind of Videos already?...
09 April 2019 2,448 8 View
Just curious if anybody here have experience with one or the other and could tell me why i should use one over the other or why i shouldnt. thanks!
04 April 2019 5,150 3 View
Suppose someone is working on molecular characterization of particular fungus using ITS region. He obtained the DNA and amplified using specific primer pairs and went for sequencing. However,...
04 April 2019 1,453 40 View
I'm a Research associate at 57357 hospital , Egypt , I would like to ask about two cell lines : RD , RH30 ( Rahbdomyosarcoma ) we couldn't find them in Egypt and we are committed to certain...
27 March 2019 1,165 4 View
I'm developing some protein surface displaying system that works in E. coli, I need to take SEM images shows successful displaying of some peptides on the surface of E. coli. However I don't...
21 March 2019 8,300 1 View
I am going to do MTT assay on HK-2 (adherent cell line) cells? I have checked many different protocols online but they are so diverse and now I am not sure which one will be suitable for my cell...
12 March 2019 1,985 3 View
Is there any report on the use of LnRNAs as gene silencing tool?
12 March 2019 7,907 7 View
03 March 2019 3,946 5 View
appeared after one week of culture , it is round and white at the at the bottom of a dish and once it appeared after one day
03 March 2019 5,407 9 View
Hi all, Am working on a DNA based biosensor, DNA are thiolated. i reduce thiolated ssDNA by TCEP and dip cast it on a gold nanoparticle coated electrode. I have tried with 500nM ,...
03 March 2019 2,913 2 View
I am at a small primarily undergraduate institution with limited resources, but we are eager to establish some important and feasible molecular/cell biology experiments on questions related to...
03 March 2019 5,669 2 View
03 March 2019 2,588 3 View
What do you know about the comet assay kits? Are they useful? How profitable are they? Is it worth buying? How was your experience with them?
03 March 2019 8,726 2 View
Hi everyone, I am newbie on using the confocal and I am wondering if the setting on all the images I take should be the same? in order to compare between them? because when I took the images I...
23 February 2019 1,920 3 View
If we have an unknown cell, for basic studies on its properties, we need to detect the combination of channels on the cell.except voltage clamp method,how we can do that? for example If we have a...
21 February 2019 9,266 4 View