Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Do you have papers on hindlimb myology of birds to share?

Hi all! we are looking for papers (or information) on muscles of the hindlimbs of birds (extant and extinct) that include the intrinsic muscles of digits. Any help will be more than welcome! Thanks!

22 February 2021 7,186 3 View

Marker Set for 3D Motion Tracking of Upper Arm during Pointing Task?

Does anyone have recommendations of a marker set for 3D motion tracking of the upper arm during a pointing task? Most studies I have come across have focused on reaching. I plan to use Vicon Nexus...

12 January 2021 3,198 1 View

Are there any way to make the comparison between different analysis of reliability possible?

10 December 2020 7,922 4 View

How do I calculate variance explained by each predictor in a multiple regression via SPSS?

I have 4 independent variables within a multiple regression model. I am trying to figure out the percentage of variance each individual independent variable contributes to the model using SPSS....

28 November 2020 583 3 View

Does anyone has suggestions to extract carbohydrates from E. coli?

Dear colleagues, We are interested in extracting carbohydrates fro E. coli and the protocol we've seen so far include too many hours in a sonicator. Does anyone has suggestions or experinece in...

26 November 2020 5,907 3 View

Is the statistical analysis performed correct?

Dear colleagues, I have some doubts about the correctness of the following analysis. A class of 79 students underwent three different teaching methods that chronologically presented themselves...

02 November 2020 4,030 4 View

Is there a validated english questionnaire to assess Achievement Motivation in Sport (Hope for Success and Fear of Failure)?

I'm looking for an instrument in english that does not measure goal orientations.

04 October 2020 3,255 5 View

How to calculate a-priori sample size for a repeated measures 2x2 ANCOVA?

I am planning an experiment 2x2 within-subjects factors and additionally I would like to consider a covariate, so that a 2x2 repeated measures ANCOVA is the intended analysis. Does anyone know how...

16 August 2020 985 4 View

How should I present microRNA expression data from a single sample (leaves)?

Hello! I have a small RNA-seq analysis from leaves, and I will validate the known and the novel miRNAs by qPCR. My question is how I should present the qPCR results from a single sample (leaves)...

30 July 2020 9,696 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View