Try to use the red channel/filter (eg. Texas Red) if it is available on the microscope.
Fluorescence Image Acquisition for BX53
1.Click the FL button on the remote controller box to use mercury arc lamp light for fluorescence. The light can be on or off by pressing FL Shutter button on the remote box.
2.Select an appropriate fluorescence mirror cube by pressing Mirror buttons in the controller box or clicking any one of the Observation Methods buttons (except BF and DIC). The selected mirror will be in position 1. Make sure that the light path bars are in correct position (the upper bar at the mid or extended position and the lower bar pushed in) and camera is selected.