Does anyone know how can I measure the reflectivity of a crystal using optical microscopy?

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Trypan Blue overestimates the viability of cryopreserved PBMC?

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How to do a systematic review?

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Maximum passage for Ewing sarcoma cell lines?

I was hoping anyone could provide any insight into using ATCC's "RD-ES" and "A-673" cell lines. How many passages do you do before you notice any senescence or genetic abnormalities

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Research about impact of brave and persistence?

I want to read about studies or experiments who proved that being brave and persistent are suceeding factors. Are there any new aspects or findings e.g. from neuroscience?

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Experience on subculturing and feeding RD-ES cell line?

Hi - I am looking for advice on protocol for subculturing and feeding RD-ES line. THey seem to grow semi-adhered and not sure if I should treat them as cells in suspension or adhered. Thank you!

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Hello everyone! Would you please help me identifying this marine polychaete ?

It was extracted from a sediment sample in the subtidal zone

19 April 2024 6,725 4 View

How to perform the embedding and cutting of 0,4 um transwell Pet inserts to visualize polarized cell layer for histology?

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How can I induce Netosis cell death in HL60 differentiated with DMSO?

Months ago i was able to induce NETosis in Hl60 differentiated cells using PMA while now with a different PMA solution I can't anymore. I followed the condition reported in literature of 100nM PMA...

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How can I extract RNA from a vine leaf?

Greetings! I started my thesis and I need to research on the methods to extract RNA from vine leaves.

05 March 2024 1,162 3 View

Could anybody help me with my human macrophages primary culture?

I am differentiating monocytes into macrophages from PBMCs, and every time I have encountered these bacillus-shaped elements when I looked them in the microscope at 40x. They are not typical...

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How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

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Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

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Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

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Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

In SEM, if a latent variable consists of three observed variables, and each observed variable has four items, what should I do?

Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...

31 July 2024 3,913 6 View

Please guide me how to prepare samples for zeiss confocal microscope?

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30 July 2024 2,349 1 View

Why Powder XRD is performed after cruhsing a crystal if Single Crystal XRD analysis of the same crystal has been performed already ?

What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?

30 July 2024 6,261 4 View

How to know the b axis of a single crystal?

How to apply magnetic field parallel to b axis of any crystal

29 July 2024 4,083 2 View