Is it ok to use mCHERRY and mCITRINE as a pair to test interactions between proteins by FRET-FLIM?

More Cécilia Cheval's questions See All

How to use the results of Co-IP for protein quantitative difference calculation?

I did a Co-IP MS with two samples and got the LFQ data from MaxQuant, and I wanted to normalize it by an internal reference protein. After I divide the LFQ of the other protein by the LFQ of the...

18 January 2021 5,419 4 View

I have tissue sections on Superfrost Slides (NOT Plus) and they fall off during IF. Any suggestion?

Hi, I have OCT embedded tissue cut on Superfrost slides. However these slides are not Superfrost Plus, so apparently they are not coated. During IF all my sections are lost. How can I save them?...

18 January 2021 5,256 2 View

Does someone had trouble with fixable life/dead in endometrial flow cytometry?

Hi. I am doing flow cytometry of endometrial cells (read epithelial and fibroblast). The antibodies I need to use are both intracellular. In the same time I want to get only life cells. Thus I...

07 January 2021 6,473 3 View

How to purify cyclic peptides?

Hello, We have synthesized the cyclic peptide (D-Glu(OtBu)-L-Lys(Alloc)-D-Glu(OtBu)-L-Ala)2. LC-MS shows a mixture of linear and cyclic peptides. There is only one peak by HPLC (reverse phase,...

21 July 2020 2,237 1 View

What are the storage conditions that prevent aggregation in B-amyloid peptids?

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07 March 2020 9,366 3 View

How can I disaggregate proteins?

I am trying to do a kinetics characterisation of AB-40 and AB-42 (they are recombinant peptides produced in the laboratory) but it seems that the samples ar alredy aggregated when I try to do the...

24 February 2020 7,437 4 View

What is the reason why poly-D-galacturonic acid is water insoluble and pectin is water soluble?

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28 October 2019 9,552 5 View

DNA precipitation without ethanol?

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22 August 2019 5,501 6 View

SDM multiple substitution?

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08 April 2019 9,620 3 View

Could you please tell me how to build Cumulative Frequency Distribution using SPSS?

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Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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03 March 2021 3,568 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

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Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

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Shall I skip the measurement model in SmartPLS?

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01 March 2021 3,052 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

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01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

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Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

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SEM or Multiple Regression for an analysis without Latent Variables?

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26 February 2021 8,399 5 View

Can anyone help with Schistosoma identification?

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25 February 2021 7,760 6 View

What's the concentration recommend for recombinant protein use in western blotting?

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24 February 2021 8,514 3 View

Harrie A Verhoeven

Dear Cécilia Cheval,

I do not think that this is the best choice you can make. Have a look at this paper:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5038762/pdf/sensors-16-01488.pdf

You will have to decide whether to use FRET on an intensity basis or in the FLIM mode. This will limit the choice of your fluoresent proteins.

I hope this is useful.Kind regards, Harrie Verhoeven.