Is it ok to use mCHERRY and mCITRINE as a pair to test interactions between proteins by FRET-FLIM?

More Cécilia Cheval's questions See All

How to use the results of Co-IP for protein quantitative difference calculation?

I did a Co-IP MS with two samples and got the LFQ data from MaxQuant, and I wanted to normalize it by an internal reference protein. After I divide the LFQ of the other protein by the LFQ of the...

18 January 2021 5,419 4 View

I have tissue sections on Superfrost Slides (NOT Plus) and they fall off during IF. Any suggestion?

Hi, I have OCT embedded tissue cut on Superfrost slides. However these slides are not Superfrost Plus, so apparently they are not coated. During IF all my sections are lost. How can I save them?...

18 January 2021 5,256 2 View

Does someone had trouble with fixable life/dead in endometrial flow cytometry?

Hi. I am doing flow cytometry of endometrial cells (read epithelial and fibroblast). The antibodies I need to use are both intracellular. In the same time I want to get only life cells. Thus I...

07 January 2021 6,473 3 View

How to purify cyclic peptides?

Hello, We have synthesized the cyclic peptide (D-Glu(OtBu)-L-Lys(Alloc)-D-Glu(OtBu)-L-Ala)2. LC-MS shows a mixture of linear and cyclic peptides. There is only one peak by HPLC (reverse phase,...

21 July 2020 2,237 1 View

What are the storage conditions that prevent aggregation in B-amyloid peptids?

We are expressing B-amyloid peptids and after purification we are having some problems with the aggregation of the samples. I'm wondering if there are optimal conditions of storage that dont...

07 March 2020 9,366 3 View

How can I disaggregate proteins?

I am trying to do a kinetics characterisation of AB-40 and AB-42 (they are recombinant peptides produced in the laboratory) but it seems that the samples ar alredy aggregated when I try to do the...

24 February 2020 7,437 4 View

What is the reason why poly-D-galacturonic acid is water insoluble and pectin is water soluble?

Pectin is mainly composed of polyagalacturonic acid (>70%) and yet is water soluble, whereas the pure acid is completely insoluble in water, even upon heating. The degree of estherification...

28 October 2019 9,552 5 View

DNA precipitation without ethanol?

Is there a way of precipitating DNA from water without using ethanol? for example, mixing DNA with glycogen and ammonium acetate and then spinning it long enough so the bound glycogen+dna would...

22 August 2019 5,501 6 View

SDM multiple substitution?

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08 April 2019 9,620 3 View

Could you please tell me how to build Cumulative Frequency Distribution using SPSS?

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15 March 2019 5,576 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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03 March 2021 3,568 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

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Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

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02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

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01 March 2021 3,052 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

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01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

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Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

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SEM or Multiple Regression for an analysis without Latent Variables?

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26 February 2021 8,399 5 View

Can anyone help with Schistosoma identification?

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25 February 2021 7,760 6 View

What's the concentration recommend for recombinant protein use in western blotting?

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24 February 2021 8,514 3 View

Harrie A Verhoeven

Dear Cécilia Cheval,

I do not think that this is the best choice you can make. Have a look at this paper:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5038762/pdf/sensors-16-01488.pdf

You will have to decide whether to use FRET on an intensity basis or in the FLIM mode. This will limit the choice of your fluoresent proteins.

I hope this is useful.Kind regards, Harrie Verhoeven.

Cécilia Cheval

Thank you Harrie, I will read this paper carefully. Kind regards. Cecilia.