Can primary dilution be done using milliQ water?

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Dörthe Andrea Kesper

Hi Viramita,

usually you can dilute your primer in sterile milliQ water - I never had problems with this.

Kind regards

Paul Rutland

I always preferred to dilute oligos in TE. The tris buffers alkaline so stops acid depurination and the edta chelates away the divalent ions present so any nucleases present in the water cannot work

Katie A S Burnette

I typically use TE buffer too. Can you ask a colleague to lend you some?

Viramita Windy

thank you for your answer